Immunoassay / Edition 1

by Eleftherios P. Diamandis, Theodore K. Christopoulos

Overview

Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians.

• Presents up-to-date information
• Provides extensive cross-referencing
• Covers theory and practice in full detail
• Written by leading authorities

Product Details

ISBN-13:	9780122147302
Publisher:	Elsevier Science
Publication date:	06/01/1996
Pages:	612
Product dimensions:	1.27(w) x 7.00(h) x 10.00(d)

About the Author

Diamandis, Eleftherios P. (Univ of Toronto); Christopoulos, Theodore K. (Univ of Windsor)

The contributors represent the specialties of membrane research, biophysics, pathology, biochemistry, and laboratory medicine. Most are from hospitals and academic medical centers in the U.S., the U.K., Canada, Spain, Germany, and Israel. Institutions prominently represented include Henry Ford Hospital, Univ of Iowa, Univ of Cincinnati, and Univ of Toronto.

Table of Contents

Past, Present, And Future of Immunological Assays. Immune Function and Antibody Structure. Theory of Immunoassays. Data Interpretation and Quality Control. Production and Purification of Antibodies. Antibody Engineering. Interferences in Immunoassays. Labeling of Antibodies and Antigens. Solid-Phases in Immunoassay. Immunoassay Configurations. The Avidin-Biotin System. Radioimmunoassay. Enzyme Immunoassay. Fluorescence Immunoassays. Chemiluminescence Immunoassay. Bioluminescence Immunoassays. Nephelometric and Turbidimetric Immunoassay. Simultaneous Multianalyte Immunoassays. Noncompetitive Immunoassay for Small Molecules. Free Hormone Measurements. Automation of Immunological Assays. Thin Film Immunoassays. Immunoblotting Techniques. Development of In-House Immunological Assays.

Editorial Reviews

Reviewer: Earle W. Holmes, PhD (Loyola University Medical Center)
Description: This multiauthored book comprises 24 chapters covering a wide range of topics in the field of immunoassay. Included are chapters devoted to antibody engineering, antibody production, labeling, each of the major classes of signal detection, automation, and immunoblotting.
Purpose: The book is written to assist in the training of individuals who wish to develop a specialist-level competency in immunodiagnostics.
Audience: The book will be of interest to clinical chemists, pathologists, and other laboratory scientists who develop immunoassays or direct immunoassay laboratories. Individual chapters stand on their own as informative reviews of selected areas of immunoassay and will be useful to students and laboratorians who are looking to learn about a specific area within the discipline.
Features: The book has a definite slant toward the diagnostic uses of immunoassay, and the authors have done an excellent job in tying the theoretical aspects to the practical issues of laboratory analysis and interpretation.
Assessment: This book provides a good breadth and depth of coverage of the subject. Many of the newer immunoassay formats and detection systems are covered, thus providing the reader with a thorough update of the development of this dynamic field. The book will be a valuable addition to the libraries of individuals who are working in the field of immunoassay or to persons who wish to attain expertise in immunodiagnostics. It is also a useful resource for libraries that serve the biomedical and life sciences research communities.

Doody's Review Service

3 Stars from Doody

This multiauthored book comprises 24 chapters covering a wide range of topics in the field of immunoassay. Included are chapters devoted to antibody engineering, antibody production, labeling, each of the major classes of signal detection, automation, and immunoblotting. The book is written to assist in the training of individuals who wish to develop a specialist-level competency in immunodiagnostics. The book will be of interest to clinical chemists, pathologists, and other laboratory scientists who develop immunoassays or direct immunoassay laboratories. Individual chapters stand on their own as informative reviews of selected areas of immunoassay and will be useful to students and laboratorians who are looking to learn about a specific area within the discipline. The book has a definite slant toward the diagnostic uses of immunoassay, and the authors have done an excellent job in tying the theoretical aspects to the practical issues of laboratory analysis and interpretation. This book provides a good breadth and depth of coverage of the subject. Many of the newer immunoassay formats and detection systems are covered, thus providing the reader with a thorough update of the development of this dynamic field. The book will be a valuable addition to the libraries of individuals who are working in the field of immunoassay or to persons who wish to attain expertise in immunodiagnostics. It is also a useful resource for libraries that serve the biomedical and life sciences research communities.

Earle W. Holmes

A thorough introduction and reference to the widely used analytic technique, balancing theory, practical considerations, quality control, automation, and subspecialties. Examines such topics as the history of the technique, immune function and antibody structure, data interpretation, antibody engineering, labeling antibodies and antigens, radioimmunoassay, free hormone measurements, and developing in-house immunological assays. For health science students, laboratory scientists, and clinicians. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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