Tumor Immunology: Methods and Protocols

by Attilio Bondanza, Monica Casucci

Overview

This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories. This book provides a better understanding of the rules governing tumor and cancer immunology, and discusses innovations in the technology of the immunological “smart bullets” (monoclonal antibodies, vaccines, tumor-reactive T cells) used to specifically target cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Tumor Immunology: Methods and Prools contains a wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

Product Details

ISBN-13:	9781493980246
Publisher:	Springer New York
Publication date:	04/14/2018
Series:	Methods in Molecular Biology , #1393
Edition description:	Softcover reprint of the original 1st ed. 2016
Pages:	165
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

ATMPs for Cancer Immunotherapy: A Regulatory Overview.- Natural Antibodies to Tumor-Associated Antigens.- Generation and Cryopreservation of Clinical Grade Wilms’ Tumor 1 mRNA-Loaded Dendritic Cell Vaccines for Cancer Immunotherapy.- Quantitative and Qualitative Analysis of Tumor Associated CD4⁺ T Cells.- Detection and Functional Analysis of Tumor-Derived LXR Ligands.- In Vitro Generation of Antigen Specific T Cells from Induced Pluripotent Stem Cells of Antigen Specific T Cell Origin.- Chimeric Ant

igen Receptors for Cancer Immunotherapy.- Cancer and Chemokines.- T Cells as Antigen-Carriers for Anti-Tumor Vaccination.- Exploiting Secreted Luciferases to Monitor Tumor Progression In Vivo.- Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models.- Modeling Human Graft-Versus-Host-Disease in Immunocompromised Mice.- ICOS Expression as Immunologic Marker in Immune Activating Monoclonal Antibodies.- Identifying NK Alloreactive Donors for Haploidentical Hematopoietic Stem Cell Transplantation.- Human Microtumors Generated in 3D: Novel Tools for Integrated In Situ Studies of Cancer Immunotherapies.