Standardization and Quality Assurance in Fluorescence Measurements I: Techniques / Edition 1

by Ute Resch-Genger

Overview

The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis.

For the first time, a team of recognized international experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field.

This first of two volumes covers basic aspects and various techniques such as steady-state and time-resolved fluorometry, polarization techniques, and fluorescent chemical sensors.

Product Details

ISBN-13:	9783540752066
Publisher:	Springer Berlin Heidelberg
Publication date:	10/10/2008
Series:	Springer Series on Fluorescence , #5
Edition description:	2008
Pages:	496
Product dimensions:	5.90(w) x 9.40(h) x 1.20(d)

Table of Contents

Need for Standardization of Fluorescence-Based Measurements.- Quantitative Fluorescence Calibration: a Tool for Assessing the Quality of Data Obtained by Fluorescence Measurements.- Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements from the View of National Metrology Institutes.- Steady State Fluorometry.- Linking Fluorometry to Radiometry with Physical and Chemical Transfer Standards: Instrument Characterization and Traceable Fluorescence Measurements.- Fluorescence Quantum Yields: Methods of Determination and Standards.- Long-Wavelength and Near-Infrared Fluorescence: State of the Art, Future Applications, and Standards.- Surface Fluorescence: the Only Standardized Method of Measuring Luminescence.- Time Resolved Fluorometry.- Time-Resolved Fluorometry: Typical Methods, Challenges, Applications and Standards.- Practical Time-Resolved Fluorescence Spectroscopy: Avoiding Artifacts and Using Lifetime Standards.- Evaluation of Time-Resolved Fluorescence Data: Typical Methods and Problems.- Time-Resolved Fluorescence: Novel Technical Solutions.- Fluorescence Polarization Techniques: Applications in the Material and the Life Sciences.- Fluorescence Depolarization Techniques in Materials Science.- Fluorescence Polarization: Recent Bioanalytical Applications, Pitfalls, and Future Trends.- Fluorescent Chemical Sensors: Principles, Problems, and Need for Quality Assurance.- Classification of Chemical Sensors and Biosensors Based on Fluorescence and Phosphorescence.- Fibre-Optic and Nanoparticle-Based Fluorescence Sensing Using Indicator Dyes: Pitfalls, Self- Referencing, Application, and Future Trends.- Intrinsically Referenced Fluorimetric Sensing and Detection Schemes: Methods, Advantages and Applications.- Total Internal ReflectionFluorescence Sensing – Quality Assurance and Application to Water Analysis.- Fluorescence Sensing and Imaging Using Pressure-Sensitive Paints and Temperature-Sensitive Paints.- Fluorescence Analysis of Actinides.- Luminescence Analysis of Actinides: Instrumentation, Applications, Quantification, Future Trends, and Quality Assurance.

Editorial Reviews

From the reviews:

"The aim of this book is to cover in considerable detail the general principles of standardization of fluorescence measurements as well as the application of these principles to both mature and cutting-edge applications. … This book is highly recommended for those who develop or utilize fluorescence-based techniques and want their measurements to be as accurate, as meaningful, and as traceable as possible." (Jonathan E. Kenny, Journal of American Chemical Society, Vol. 131 (10), 2009)

From the Publisher