Single Cell Diagnostics: Methods and Protocols / Edition 1

by Alan R. Thornhill

Overview

The aim of Single Cell Diagnostics: Methods and Prools is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

Product Details

ISBN-13:	9781588295781
Publisher:	Springer-Verlag New York, LLC
Publication date:	06/15/2007
Series:	Methods in Molecular Medicine , #132
Edition description:	2007
Pages:	182
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Noninvasive Metabolic Assessment of Single Cells.- Laser Capture Microdissection for Analysis of Single Cells.- Fluorescence In Situ Hybridization on Single Cells.- Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis.- Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma.- Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification.- Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies.- Efficient Isothermal Amplification of the Entire Genome from Single Cells.- Comparative Genomic Hybridization on Single Cells.- Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells.- Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells.- Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization.- Microarray Technology for Mutation Analysis of Low-Template DNA Samples.

Editorial Reviews

Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. The 13 chapters cover topics ranging from laser capture microdissection to fluorescence in situ hybridization on single cells to use of real time PCR to measure gene expression in single cells. The experimental protocols described in this book were developed initially to examine the blastomeres of human embryos for single gene mutations and chromosomal abnormalities. The primary focus of this book is on biochemistry and molecular biology.
Purpose: According to the editor, the aim "is for all readers to extend their knowledge and expertise in analysis of single cells." The authors detail methods for salvaging diagnostic information from single cells without contamination. The authors believe that this book will provide an excellent source of tips for diagnostic medicine, reproductive medicine, and experimental biology.
Audience: The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. The editor and authors believe that the technology outlined in this book will become incorporated into routine diagnostic procedures for a wide spectrum of human conditions and diseases, including neoplasia.
Features: Each chapter provides a summary, introduction, list of materials, and detailed outline of experimental protocols. Notes at the end of each chapter provide tips that are an invaluable source of practical information. The authors provide examples of interesting results from their laboratories. The chapters are written by experts and the book is carefully edited. Line drawings and continuous tone figures primarily serve to illustrate conceptual points.
Assessment: This concise book is written for experts with a sophisticated knowledge of cell and molecular biology. The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics. Future applications of this technology may revolutionize experimental biology, allowing researchers to analyze patterns of protein and gene expression in individual cells in developing embryos and rare adult tissues.

Doody's Review Service

From the reviews:

"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. … For researchers this book provides not only detailed state of the art prools for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. … This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)

"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. … The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. … The chapters are written by experts and the book is carefully edited. … The laboratory prools and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)

From the Publisher