Pyrosequencing Protocols / Edition 1

by Sharon Marsh

Overview

Pyrosequencing® Prools presents detailed prools for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each prool with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed prools for the application of Pyrosequencing®.

Product Details

ISBN-13:	9781617376931
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/01/2010
Series:	Methods in Molecular Biology , #373
Edition description:	Softcover reprint of hardcover 1st ed. 2007
Pages:	196
Product dimensions:	5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

The History of Pyrosequencing®.- Pyrosequencing® Applications.- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing®.- Pyrosequencing®.- Universal Primer Applications for Pyrosequencing®.- Allele Quantification and DNA Pooling Methods.- Multiplex Pyrosequencing® for DNA Variation Analysis.- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology.- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria.- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci.- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands.- Gene Copy Number Detection in Animal Studies.- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing®.- Pyrosequencing® Genotype Storage Techniques.