Phospholipid Signaling Protocols / Edition 1

by Ian Bird

Overview

Cell membranes are not, as once believed, inert structures designed to contain the cell contents, but are in fact dynamic structures that are as me- bolically active as the cytosol and other cellular compartments they surround. Thus membranes not only contain mixtures of lipid and phospholipids, but also many proteins both embedded deeply within the membrane structure itself and also more loosely attached on the membrane surfaces. Though many such proteins have long been known to act as transport proteins, ion channels, hormone receptors, G proteins, cytoskeletal anchorage points, and so on, the major advance of recent years is the increasing understanding that the lipids and phospholipids in the membrane bilayer itself are also metabolized to b- logically active products that can diffuse either in the cytosol or in the m- brane bilayer to control the function of other proteins. Thus the concept of lipid-derived second messengers is now firmly established.

Product Details

ISBN-13:	9780896034914
Publisher:	Springer-Verlag New York, LLC
Publication date:	04/15/1998
Series:	Methods in Molecular Biology , #105
Edition description:	1998
Pages:	380
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

About the Author

Bird, Ian M. (Univ of Wisconsin)

Table of Contents

Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways.- Phosphoinositidase C Activation Assay I.- Phosphoinositidase C Activation Assay II.- Phosphoinositidase C Activation Assay III.- Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection.- Preparation of [3H]Phosphoinositol Standards and Conversion of [3H]Phosphoinositides to [3H]Phosphoinositols.- Inositol 1,4,5-Trisphosphate Mass Assay.- Measurement of Cellular Diacylglycerol Content.- Phosphatidylinositol 4-Kinases.- Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay.- Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography.- Determination of Phospholipase C-or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis.- Measurement of Phospholipase D Activity.- Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid.- Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases.- Isotopic Efflux Studies as Indices of Phospholipase Activation.- Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays.- Measurements of Prostanoids, Leukotrienes, and Isoprostanes by Enzyme Immunoassays.- Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways by High-Pressure Liquid Chromatography.- Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis.- Sphingomyelin and Ceramide Mass Assay.- Sphingosine Kinase.- Analytical Methods and Steps to Sample Preparation for Determination of Molecular Species of Fatty Acids.- HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids in Diacylglycerol and Cellular Phospholipids.- Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides.- General Methods for Monitoring Changes in Levels of Key Signaling Pathway Proteins and Associated mRNA.- Solubilization and Assay of Cellular and Tissue Protein.- Western Immunoblot Analysis.- Immunohishemistry and Immunocyhemistry.- Extraction of Cellular and Tissue RNA.- Size Separation and Quantification of mRNA by Northern Analysis.- Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR.- Optimization of a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Mass Assay for Low-Abundance mRNA.

Editorial Reviews

Contributors detail techniques for monitoring the major lipid and phospholipid-derived second messenger pathways to identify and quantify pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and its associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase and sphingosine kinase activity, and for the quantification, separation, and rigorous identification of phospholipids, diacylglycerol, and sphingolipids, as well as their metabolites. There is also extensive information on the extraction, size separation, detection, and quantification of cellular signaling proteins and corresponding mRNA. Annotation c. by Book News, Inc., Portland, Or.

Booknews

". . . the detailed information provided in Phospholipid Signaling Prools makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams

". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several prools in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology

"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important prools together..."-Cell Biology International 2000

"...does a very good job of covering what is a fairly diverse field and the prools described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology

"...does a very good job covering what is a fairly diverse field and the prools described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology

From the Publisher