Neurochemistry: A Practical Approach / Edition 2

by A. J. Turner, H. S. Bachelard

Overview

Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervous system, chemical neuroanatomy, and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABA transporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.

Product Details

ISBN-13:	9780199634392
Publisher:	Oxford University Press
Publication date:	02/20/1997
Series:	Practical Approach Series , #172
Edition description:	REV
Pages:	322
Product dimensions:	6.19(w) x 9.25(h) x 0.67(d)

About the Author

Turner, A. J. (Univ of Leeds); Bachelard, H. S. (Univ of Nottingham)

The contributors represent the specialties of neurobiology, neurochemistry, physiology, pharmacology, and biochemistry. Most are from universities and academic medical centers in the U.K., the U.S., and Russia. Institutions prominently represented include Univ of Kent, Institute of Neurology-London, Univ of Newcastle, and Univ of Kentucky.

Table of Contents

1. Isolation of synaptosomes, growth cones and their subcellular components2. Proteins of the neuronal cytoplasmic and membrane-associated cytoskeleton3. Immunocytochemical techniques for the study of the nervous system4. In situ hybridization approaches to the study of the nervous system5. Investigation of mitochondrial defects in brain and skeletal muscle6. Measurement of neurotransmitters and their associated second messengers in vivo7. Protein phosphorylation and dephosphorylation in the nervous system8. Receptor-linked phosphoinositide metabolism9. Neurotransmitter transporters: purification, reconstitution, and assay of the brain GABA transporter

Editorial Reviews

3 Stars from Doody

This second edition maintains the high standards of other volumes in this series. The purpose of this series is to provide a ""how to"" manual for laboratory scientists in basic science areas fundamental to the neurosciences. The editors have rightly deleted material on cell cultures, molecular neurobiology, and neuroreceptors because these areas have grown to require separate volumes of their own. The volume describes valuable procedures for fractionating and analyzing subcellular component of the nervous systems, visualizing neuronal macromolecules, and measuring neuronal metabolism in vivo and in vitro. The editors intended this volume to help postgraduate and postdoctoral students as well as established researchers who have switched into the neurosciences. However these protocols, especially those for investigating mitochondrial defects in brain, will be of value to those already in the field. The protocols are detailed and clear as would be expected by the authorities selected to write each chapter. This volume will have a broad appeal to basic scientists who conduct the procedures indicated by the protocols, medical and graduate students, and clinicians who are interested in exactly how the experiments are conducted. Of particular interest to clinicians is the amount of disarticulation of cytoarchitecture that occurs in sample preparations. The isolation of synaptosomes and membrane associated cytoskeleton are examples of this difficulty in attaining purity. Many excellent illustrations and graphs are provided to illustrate the text. Each protocol is set off with a box with numerical divisions and reagents specified and rules for each step in the procedure. This volumeprovides specific protocols for fractionating subcellular components such as synaptosomes, growth cones, microtubules, membrane-associated cytoskeleton components, neurofilaments, and calmodulin. Other chapters include immunocytochemical techniques, in situ hybridization, mitochondrial biochemistry, measurement of neurotransmitters and their associated second messengers in vivo, protein phosphorylation, neurotransmitter transporter purification, and receptor-linked phosphoinositide metabolism. Each chapter is well written and concisely presented by authorities in their field. These techniques are especially valuable for guiding actual experiments and for those who want to know how such experiments are performed.

Thomas H. Jobe

Reviewer: Thomas H. Jobe, MD (University of Illinois at Chicago College of Medicine)
Description: This second edition maintains the high standards of other volumes in this series. The purpose of this series is to provide a "how to" manual for laboratory scientists in basic science areas fundamental to the neurosciences. The editors have rightly deleted material on cell cultures, molecular neurobiology, and neuroreceptors because these areas have grown to require separate volumes of their own. The volume describes valuable procedures for fractionating and analyzing subcellular component of the nervous systems, visualizing neuronal macromolecules, and measuring neuronal metabolism in vivo and in vitro.
Purpose: The editors intended this volume to help postgraduate and postdoctoral students as well as established researchers who have switched into the neurosciences. However these protocols, especially those for investigating mitochondrial defects in brain, will be of value to those already in the field. The protocols are detailed and clear as would be expected by the authorities selected to write each chapter.
Audience: This volume will have a broad appeal to basic scientists who conduct the procedures indicated by the protocols, medical and graduate students, and clinicians who are interested in exactly how the experiments are conducted. Of particular interest to clinicians is the amount of disarticulation of cytoarchitecture that occurs in sample preparations. The isolation of synaptosomes and membrane associated cytoskeleton are examples of this difficulty in attaining purity.
Features: Many excellent illustrations and graphs are provided to illustrate the text. Each protocol is set off with a box with numerical divisions and reagents specified and rules for each step in the procedure.
Assessment: This volume provides specific protocols for fractionating subcellular components such as synaptosomes, growth cones, microtubules, membrane-associated cytoskeleton components, neurofilaments, and calmodulin. Other chapters include immunocytochemical techniques, in situ hybridization, mitochondrial biochemistry, measurement of neurotransmitters and their associated second messengers in vivo, protein phosphorylation, neurotransmitter transporter purification, and receptor-linked phosphoinositide metabolism. Each chapter is well written and concisely presented by authorities in their field. These techniques are especially valuable for guiding actual experiments and for those who want to know how such experiments are performed.

Doody's Review Service