Neural Cell Transplantation: Methods and Protocols / Edition 1

by Neil J. Scolding, David Gordon

Overview

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Prools, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Prools provides the most thorough and essential prools that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

Product Details

ISBN-13:	9781603279307
Publisher:	Springer-Verlag New York, LLC
Publication date:	05/07/2009
Series:	Methods in Molecular Biology , #549
Edition description:	2009
Pages:	226
Product dimensions:	7.70(w) x 10.30(h) x 0.80(d)

Table of Contents

Introductory Reviews.- Neural Transplantation and Stem Cells.- Adult Stem Cells for the Treatment of Neurological Disease.- Human Trials for Neurodegenerative Disease.- Practical/Methodological Chapters.- Differentiation of Neuroepithelia from Human Embryonic Stem Cells.- Derivation of High-Purity Oligodendroglial Progenitors.- Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny.- Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay.- Human Mesenchymal Stem Cell Culture for Neural Transplantation.- Umbilical Cord Blood Cells.- Animal Models of Neurodegenerative Diseases.- Animal Models of Multiple Sclerosis.- Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies.- A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery.- MR Tracking of Stem Cells in Living Recipients.- Identifying Neural Progenitor Cells in the Adult Brain.- Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of Poor Prognosis Multiple Sclerosis.

Editorial Reviews

Reviewer: Celso Agner, MD, MS, MSc (Michigan Neurology Partners)
Description: The field of molecular biology has dramatically changed perspectives on the management of degenerative diseases of the central nervous system. In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS.
Purpose: The main purpose is to provide, in a stepwise fashion, the methods involved in the production of stem cell lines and transplantation into the central nervous system.
Audience: Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. The authors are recognized experts in the field.
Features: The introductory chapters summarize where we are in terms of stem cells and what aspects should be considered in their preparation prior to transplantation. However, one of the major drawbacks of these first chapters is the lack of a profound analysis of the ethical and religious concerns associated with human stem cells and what alternatives, including omental cells, could be considered to circumvent the limitations of available tissue. Otherwise, the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs involved in the research and clinical applications of the technology, in particular for degenerative conditions such as Alzheimer's disease, Parkinson's disease, multiple sclerosis, and others. Another drawback is the brief description of the clinical applications in contrast to the extensive description of the protocols. As a result, the book is a comprehensive reference for neuroscientists, but not so much for clinicians.
Assessment: This is a good book for neuroscientists who are directly involved in the separation and production of human stem cells.

Doody's Review Service

From the reviews: “In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS. … Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. … the book flows easily and describes the most common research and technical prools associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs … . a good book for neuroscientists who are directly involved in the separation and production of human stem cells.” (Celso Agner, Doody’s Review Service, December, 2009)

From the Publisher