Molecular Analysis of Cancer / Edition 1

by Jacqueline Boultwood, Carrie Fidler

Overview

Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluor- cent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a pro- nent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Prools for the detection of chromosomal re- rangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis.

Product Details

ISBN-13:	9780896036222
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/10/2001
Series:	Methods in Molecular Medicine , #68
Edition description:	2002
Pages:	304
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

About the Author

Boultwood, Jacqueline; Fidler, Carrie

Table of Contents

Molecular Analysis of Cancer.- Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization.- Spectral Karyotyping in Cancer Cytogenetics.- Comparative Genomic Hybridization Analysis.- Detection of Chromosomal Deletions by Microsatellite Analysis.- Detection and Quantification of Leukemia-Specific Rearrangements.- Detection of t(2;5)(p23;q35)Translocation by Long-Range PCR of Genomic DNA.- Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer.- Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism.- Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis.- Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay.- Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay.- Mutation Analysis of Cancer Using Automated Sequencing.- Detection of Differentially Expressed Genes in Cancer Using Differential Display.- Genomewide Gene Expression Analysis Using cDNA Microarrays.- Gene Expression Profiling in Cancer Using cDNA Microarrays.- Wilms Tumor Gene WT1 as a.- Detection of Aberrant Methylation of the p15 INK4B Gene Promoter.- Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon.- 20Telomere Length Changes in Human Cancer.- Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders.

Editorial Reviews

Reviewer: Jochen H. Lorch, MD (Northwestern University Feinberg School of Medicine)
Description: This is a short, concise and surprisingly comprehensive manual with many useful hints.
Purpose: The book is a short manual describing techniques for identification of chromosomal abnormalities. These objectives are worthy and the objectives have been met.
Audience: The manual should be useful for students and scientists alike.
Features: The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher. I liked the rather extensive bibliography at the end of each chapter. Due to the limited scope of the book, some newer techniques such as microarrays are not covered
Assessment: A useful little book which should provide good guidance for the young researcher at the beginning of his or her career. It can be a useful addition to the more extensive standard lab manuals such as Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, 2001) and others.

Doody's Review Service

"The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher." -Doody's Health Sciences Book Review Journal

"This book gives a good overview of currently available methods for investigating molecular changes in cancers,...it illustrates how you, the cancer researcher, can apply the methods to your oncology problem...The most enjoyable chapters also illustrate the methods with real results, so that you can see what to expect, e.g. chapter 3 on spectral karyotyping...a useful addition in any oncology research laboratory, because it presents this large variety of methods in a compact format, so that someone waiting to try a new approach could easily dip into the book to see what is available." - Molecular Biotechnology

"Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities." - Clinical Laboratory

"...good integration between preliminary explanations of techniques and practical instructions with easily available tables or figures." - Proteomics

From the Publisher

3 Stars from Doody