Forced-Flow Layer Chromatography

by Erno Tyihak

Overview

Forced-Flow Layer Chromatography takes a close look at the specifics of forced-flow layer chromatography techniques, from their evolution to the nuances of using these techniques in a variety of applications where traditional thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are not as effective.

This book presents a number of variations of TLC techniques, with special emphasis on the overpressured-layer chromatography (OPLC) technique and newer developments such as the BioArena System for biomedical analysis. The versatility of these forced-flow techniques opens up new avenues for the analysis of a large number of samples for high-throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis.

Product Details

ISBN-13:	9780124201613
Publisher:	Elsevier Science
Publication date:	12/02/2015
Pages:	524
Product dimensions:	7.50(w) x 9.25(h) x (d)

About the Author

Dr. Erno Tyihák’s primary scientific achievements include the discovery of the formaldehyde cycle and formaldehydome system, the quadruple immune response of plants to pathogens, the unique role of endogenous formaldehyde and ozone in the antibiotic effect and the double effect of trace elements similar to trans-resveratrol. He was an early adopter of thin-layer chromatography methods and has been instrumental in moving the field forward, ultimately developing different patented versions of overpressured-layer chromatography, widely known as one of the most efficient separation techniques. Most recently he has invented the BioArena system, which integrates layer chromatographic separation with biological detection capabilities to explore in vivo phenomena such as the immunization of plants in greenhouse conditions. He holds PhD and DSc degrees from the Hungarian Academy of Sciences. He is Honorary Ass. Professor at the Semmelweis University, Budapest, Hungary, as well as Honorary Professor at the University of Szeged, Szeged, Hungary, and he was until recently a Scientific Advisor at the Plant Protection Institute, Hungarian Academy of Sciences Budapest, Hungary.

Table of Contents

1. Unique potentialities of layer liquid system - results, limitations, new demands Erno Tyihák 2. Overpressured-layer chromatography Erno Tyihák, Ágnes M. Móricz, and Emil Mincsovics 3. Centrifugal layer chromatography - Rotation planar chromatography Ágnes M. Móricz and Irena Vovk 4. Planar chromatography using electroosmotic flow Aneta Halka-Grysinska, Radoslaw L. Gwarda, Tadeusz H. Dzido 5. Layer dielectrochromatography - under external alternating current, LDEC Virginia Coman and Stefan Kreibik 6. Conventional and modern bioassays - detection, isolation, identification Ágnes M. Móricz and Péter G. Ott 7. BioArena system for studying key molecules as well as ingredients in biological samples Erno Tyihák and Ágnes M. Móricz 8. Determining progress directions in layer liquid chromatography - dreams and realities Erno Tyihák

What People are Saying About This

From the Publisher

A review of the basic elements, separation methodologies, and biological detection potential of forced-flow layer chromatography, with special emphasis on overpressured layer chromatography

Editorial Reviews

"...provides a comprehensive overview of the different approaches in FFLC, making it a valuable resource for everyone studying planar chromatography and its potential." —Analytical and Bioanalytical Chemistry

From the Publisher