Enzyme and Microbial Biosensors: Techniques and Protocols / Edition 1

by Ashok Mulchandani, Kim Rogers

Overview

In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form “enzyme el- trodes” in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a biosensor. The purpose of the two volumes of Prools and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. There are a variety of approaches that researchers employ to select a combination of bioaffinity elements and signal transducers. One commonly used approach is to identtfy the compound or compounds of interest; identify the biological molecule that yields an appropriate recognitionlselectivtty and dynamic concentration range for the assay; and choose an assay format and signal transduction technology that will meet the analytical requirements for the proposed application, This volume, Enzyme and Microbial Biosensors: Techniques and Prools, describes a variety of transduction technologies that have been interfaced to enzymes and microorganisms.

Product Details

ISBN-13:	9780896034105
Publisher:	Springer-Verlag New York, LLC
Publication date:	04/17/1998
Series:	Methods in Biotechnology , #6
Edition description:	1998
Pages:	264
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

About the Author

Mulchandani, Ashok (UC Riverside); Rogers, Kim R. (U.S. Environmental Protection Agency)

Table of Contents

Enzyme Biosensors.- Principles of Enzyme Biosensors.- Enzyme Biosensors Based on pH Electrode.- Enzyme Biosensors Based on Gas Electrodes.- Enzyme Biosensors Based on ISFETs.- Enzyme Biosensors Based on Oxygen Detection.- Enzyme Biosensors Based on the Hydrogen Peroxide Electrode.- Enzyme Biosensors Based on Mediator-Modified Carbon Paste Electrode.- Enzyme Biosensors Based on Electron Transfer Between Electrode and Immobilized Peroxidases.- Enzyme Biosensors Based on Redox Polymers.- Enzyme Biosensors Based on Metallized Carbon Electrodes.- Enzyme Biosensors Based on Conducting Polymers.- Enzyme Sensors Based on Conductimetric Measurement.- Enzyme Biosensors Based on Thermal Transducer/Thermistor.- Enzyme Biosensors Based on Fluorometric Detection.- Microbial Biosensor.- Microbial biosensors based on oxygen electrodes.- Microbial biosensors based on respiratory inhibition.- Microbial biosensors based on potentiometric detection.- Microbial biosensors based on optical detection.

Editorial Reviews

Reviewer: Howard H. Sky-Peck, MD, PhD, FRSM (Rush Medical College of Rush University)
Description: This volume, Number 6 in the Methods in Biotechnology series, provides a description of a variety of transduction techniques that have been interfaced to enzymes and microorganisms.
Purpose: The purpose is to provide a basic reference tool and starting point to enable rapid entry into the field of biosensors.
Audience: It is written to introduce graduate and postdoctoral students and senior researchers to the field of biosensors.
Features: Although not an exhaustive treatise, this book provides a detailed, step-by-step description for a variety of enzyme- and microbial-based biosensor techniques that will allow a novice or an experienced investigator to expand into new areas of research most appropriate for their analytical needs. The illustrations are simple and helpful, and the references are up-to-date and extensive.
Assessment: This is a very useful volume for the detailed methodologies related to biosensors for use in analytical techniques. This volume is primarily devoted to theory and mechanisms of the technique, while the subsequent volume presents methodologies. This subsequent volume is reviewed separately.

Doody's Review Service

3 Stars from Doody

This volume, Number 6 in the Methods in Biotechnology series, provides a description of a variety of transduction techniques that have been interfaced to enzymes and microorganisms. The purpose is to provide a basic reference tool and starting point to enable rapid entry into the field of biosensors. It is written to introduce graduate and postdoctoral students and senior researchers to the field of biosensors. Although not an exhaustive treatise, this book provides a detailed, step-by-step description for a variety of enzyme- and microbial-based biosensor techniques that will allow a novice or an experienced investigator to expand into new areas of research most appropriate for their analytical needs. The illustrations are simple and helpful, and the references are up-to-date and extensive. This is a very useful volume for the detailed methodologies related to biosensors for use in analytical techniques. This volume is primarily devoted to theory and mechanisms of the technique, while the subsequent volume presents methodologies. This subsequent volume is reviewed separately.

Howard H. Sky-Peck

Detailing methods developed in their own labs, contributors provide protocols for enzyme biosensors based on potentiometric, amperometric, conductimetric, optical, and thermal transducers; and microbial biosensors based on oxygen-gas electrode and optical techniques in which microorganisms are coupled to the signal transducer. Chapter one provides an overview of the principles relevant to the design and operational features of enzyme-based biosensors. The contents of the companion volume, are appended. Annotation c. by Book News, Inc., Portland, Or.

Booknews

"Mulchandani and Rogers have assembled an international group of authors that presents a comprehensive panel of biosensors. . .the consistent format among chapters is useful and refreshing. . .chapters include concise descriptions of the fundamentals and developmental history of the highlighted transducer. . .every chapter includes tips for ensuring experimental success."-Molecular Biotechnology

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