Comprehensive Enzyme Kinetics

by Vladimir Leskovac

Overview

This text for advanced courses in enzyme chemistry and enzyme kinetics covers the field of steady-state enzyme kinetics from the basic principles inherent in the Michaelis-Menten equation to expressions that describe the multi-substrate enzyme reactions, providing a framework for the study of enzymes with the aid of kinetic studies of enzyme-catalyzed reactions. Discussion encompasses chemical kinetics, kinetics of monosubstrate reactions, and cooperative and allosteric effects. The editor is affiliated with the University of Novi Sad. Annotation ©2003 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9780306467127
Publisher:	Springer US
Publication date:	03/31/2003
Edition description:	2003
Pages:	438
Product dimensions:	6.14(w) x 9.21(h) x 0.36(d)

Table of Contents

1. Introduction. 2. Chemical kinetics. 3. Kinetics of monosubstrate reactions. 4. Derivations of rate equations. 5. Linear inhibition. 6. Hyperbolic and parabolic inhibition. 7. Enzyme activation. 8. Kinetics of rapid equilibrium bisubstrate reactions. 9. Steady state kinetics of bisubstrate reactions. 10. Kinetic analysis of bisubstrate mechanisms. 11. Substrate inhibition and mixed dead-end and product inhibition. 12. Kinetics of trisubstrate reactions. 13. Cooperative and allosteric effects. 14. The pH dependence of enzyme catalysis. 15. Effects of temperature on enzyme reactions. 16. Isotope exchange. 17. Solvent and kinetic isotope effects. 18. Statistical analysis of initial rate and binding data. Subject index.

Editorial Reviews

‘As scientific attention turns in the post-genomic era, once again, to the properties of proteins, and particularly to their preeminent role as catalysts, Professor Leskovac's book, Comprehensive Enzyme Kinetics, will have to be on every shelf. It will, however, rarely find itself on the shelf, but much more in the hands of researchers who will daily deploy in the laboratory its wealth of useful information. Prepared under the eye of W.W. Cleland, the demiurge of enzyme kinetics, this magnum opus earns its title of comprehensive. The systematic organization of the volume, which somehow manages completeness in just over 400 pages, leads the reader to a target subject easily. The clarity of exposition will be as welcome to the experienced kineticist as it will be to the beginner. This volume is a singular achievement.'
R.L. Schowen, Professor Emeritus Chemistry, Molecular Biosciences and Pharmaceutical Chemistry, University of Kansas
‘Comprehensive Enzyme Kinetics is an excellent addition to the enzyme kinetics literature. It touches on all of the topics in the various areas of kinetics that might be of interest to the enzymologist. It will make a fine classroom reference text.'
Herbert J. Fromm, Distinguished Professor, Iowa State University

From the Publisher