Cell Imaging Techniques: Methods and Protocols / Edition 2

by Douglas J. Taatjes, Jürgen Roth

Overview

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Prools expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls

Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Prools, Second Edition is an easily accessible volume of prools to be used with a variety of imaging-based equipment likely available in a core imaging facility.

Product Details

ISBN-13:	9781627030557
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/02/2012
Series:	Methods in Molecular Biology , #931
Edition description:	2nd ed. 2013
Pages:	550
Product dimensions:	7.01(w) x 10.00(h) x 0.06(d)

Table of Contents

Digital Images are Data – and Should be Treated as Such.- Epi-fluorescence Microscopy.-Live-Cell Migration and Adhesion Turnover Assays.-Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in Human Venous Valve.-Colocalization Analysis in Fluorescence Microscopy.-A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown.-Light Sheet Microscopy in Cell Biology.-Image-based High-throughput Screening for Inhibitors of Angiogenesis.-Intravital Microscopy to Image Membrane Trafficking in Live Rats.-Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent.-Laser Scanning Cytometry. Principles and Applications: An Update.-Laser Capture Microdissection for Protein and NanoString RNA Analysis.-Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy.-Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.-Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation.-Colocalization Analysis in Fluorescence Microscopy.-A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown.-Light Sheet Microscopy in Cell Biology.-Image-based High-throughput Screening for Inhibitors of Angiogenesis.-Intravital Microscopy to Image Membrane Trafficking in Live Rats.-Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent.-Laser Scanning Cytometry. Principles and Applications: An Update.-Laser Capture Microdissection for Protein and NanoString RNA Analysis.-Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy.-Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.-Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation.-Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells.-Porosome: The Secretory NanoMachine in Cells.-Stereology andMorphometry of Lung Tissue.-A Novel Combined Imaging/Stereological Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis.-Correlative Light–Electron Microscopy as a Tool to Study In vivo Dynamics and uUtrastructure of Intracellular Structures.-Photooxidation Technology for Correlative Light and Electron Microscopy.-Electron Microscopy of Endocytic Pathways.-Morphological Analysis of Autophagy.-Cyhemical Detection of Peroxisomes and Mihondria.-Hishemical Detection of Lipid Droplets in Cultured Cells.-Environmental Scanning Electron Microscopy in Cell Biology.-Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology.-High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles.-MALDI Imaging Mass Spectrometry for Direct Tissue Analysis.

Editorial Reviews

Reviewer: Latha Malaiyandi, PhD (Midwestern University)
Description: This update of a compilation of microscopy protocols reflecting the evolving advances in cellular imaging is a considerable overhaul of the 2006 edition and includes 26 new chapters.
Purpose: The goal is to provide seasoned microscopists with a list of protocols to add sophistication to their existing microscopy regimen.
Audience: The intended audience is experienced researchers who are familiar with imaging in a core microscopy facility. In contrast to a beginner's book for learning microscopy, this book is more useful to microscopists who routinely use microscopic techniques in their research and are knowledgeable about the recent advances in technology.
Features: Each chapter is written by a different group of researchers who have expertise in microscopic techniques, such as epi-fluorescence, confocal, laser scanning, atomic force, and scanning electron microscopy. In addition, these techniques are performed using many different types of specimens - ranging from single molecules to arterioles or lung tissue to even whole animals. The applications are also very specific. For example, the book describes assays to study live cell migration and adhesion turnover, time-lapse imaging of nuclear envelope breakdown, intravital microscopy of membrane trafficking in live rats, etc. Each chapter introduces the technique and under what conditions it may be used, a very detailed protocol including the exact instruments, materials, and reagents required, expected traces and images, and appropriate methods for image processing. The authors also include caveats (in the form of "Notes") for artifacts that emerge from certain conditions or reagents. Each chapter concludes with an exhaustive list of references. The techniques are highly specific and written for researchers who have prior knowledge of advanced microscopy.
Assessment: This is an ideal reference for a core microscope facility. Each chapter is written independently by a different group of researchers and can stand alone. The protocols are very detailed and describe techniques on a step-by-step basis, but assume that readers have the specific instruments and materials to carry them out. A unique feature of the book is an introductory chapter on the history of data manipulation that offers guidelines on how to avoid image alteration and interpret the science.

Doody's Review Service

From the book reviews:

“The different techniques are described in deep details, the chapters having an abstract, introduction, materials, methods, and, last but not least, notes; each method is described step by step with very, very useful links to the notes. … should be read not only by very specialized scientists, but also by those who usually work with classic microscopic methods, to open their minds and increase their appetite for more precise and complex measurements done on different level of complex biological structures.” (Ioan I. Ardelean, Bulletin of Micro and Nanoelectrotechnologies, Vol. 5 (3-4), December, 2014)

“The goal is to provide seasoned microscopists with a list of prools to add sophistication to their existing microscopy regimen. The intended audience is experienced researchers who are familiar with imaging in a core microscopy facility. … this book is more useful to microscopists who routinely use microscopic techniques in their research and are knowledgeable about the recent advances in technology. … an ideal reference for a core microscope facility. Each chapter is written independently by a different group of researchers and can stand alone.” (Latha Malaiyandi, Doody’s Book Reviews, December, 2013)

From the Publisher