Bacterial Artificial Chromosomes: Volume 2: Functional Studies / Edition 1

by Shaying Zhao, Marvin Stodolsky

Overview

Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970–1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so that they substantially replaced the YACs used earlier. Much of the early automation for massive clone picking and processing was developed at the collaborating Lawrence Berkeley National Laboratory. However, the P1 virion encapsidation system itself was too fastidious, and P1 virion-based methods did not gain popularity in other genome projects.

Product Details

ISBN-13:	9780896039896
Publisher:	Springer-Verlag New York, LLC
Publication date:	03/04/2004
Series:	Methods in Molecular Biology , #256
Edition description:	2004
Pages:	326
Product dimensions:	6.10(w) x 9.30(h) x 1.10(d)

Table of Contents

Use of BAC End Sequences for SNP Discovery.- Exon Trapping for Positional Cloning and Fingerprinting.- Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column.- BAC Microarray-Based Comparative Genomic Hybridization.- Large DNA Transformation in Plants.- Retrofitting BACs With a Selectable Marker for Transfection.- BAC Modification Using a RecA Expressing Shuttle Vector System.- Bacterial Artificial Chromosome Engineering.- ET Recombination.- BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes.- Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes.- Generation of BAC Transgenic Mice.- BAC Rescue.- Herpesviruses.- Cloning of—-Herpesvirus Genomes as Bacterial Artificial Chromosomes.- Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors.- Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination).- Mutagenesis of Herpesvirus BACs by Allele Replacement.- Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies.- Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I.