Applications of Genomics and Proteomics for Analysis of Bacterial Biological Warfare Agents

by V.G. DelVecchio, V. Krcmery

Overview

Recent scientific breakthroughs have improved our understanding of the impact of environmental toxic substances on human health. With the knowledge, new techniques are incorporated into human monitoring programs to identify damage to DNA, other macromolecules and cells that can be used to predict health risk from exposure to environmental genotoxic agents. The molecular and cellular responses are often measured in peripheral lymphocytes because these cells can be collected in a socially and ethically acceptable manner, and via a minimally invasive route. However, other cells such as sperm, buccal or nasal epithelial cells, placental cells, and body fluids such as urine and serum, are also used. From these investigations, it becomes obvious that an individual's genetic constitution and lifestyle activities can significantly influence the exposure effect. Genetic constitution includes inherited variations in genes for chemical metabolism and DNA repair. Lifestyle activities include diet and levels of physical activity. Because of the significant influence of the environmental factors on human health, there is increased interest in monitoring human populations for improvement of health. The major objective of this publication is to enhance the knowledge and expertise of researchers in molecular epidemiology and human monitoring.

Product Details

ISBN-13:	9781586033439
Publisher:	IOS Press, Incorporated
Publication date:	01/01/2003
Series:	NATO Science Series
Pages:	196
Product dimensions:	9.66(w) x 6.52(h) x 0.69(d)

Table of Contents

	Preface and Acknowledgments	v
	Organizing Committee	vi
	Key Speakers	vii
	Participants and Observers	x
	Bacterial Biological Warfare	1
	Brucella melitensis Genome: Hidden Credentials for Virulence	7
	Genome Analysis of Francisella Specific Plasmids	15
	Principles and Applications of Proteomics in Brucella Research	25
	Proteome of Francisella tularensis Microbe	43
	Bacillus anthracis Secretome	53
	Future Directions in BWA Detection and Diagnosis	67
	Antibiotic Resistance and Bioterrorism	71
	Molecular Epidemiology of Antibiotic-resistant Bacteria	75
	Gene Transfer in Enterobacterial Pathogens	83
	SSH Subtractive Cloning: Comprehensive Survey of Genome Differences in Prokaryotes	95
	Rapid Fingerprinting and Diagnostic Assays for Biological Warfare Agents	105
	Bacterial Artificial Chromosome Libraries of Antibiotic-producing Actinomycetes	111
	History and Future of Brucellosis Vaccine Development	123
	History and Future of Vaccine Development against Anthrax	129
	Mass Spectrometry Approaches in Protein Interaction Studies	135
	Determination and Structural Examination of Potential Biomarkers for Methicillin-resistant Staphylococcus aureus	149
	Microparticulated Rough Subcellular Vaccine against Brucellosis	155
	Poster Abstracts	163
	Future Directions	175
	Subject Index	177
	Author Index	179