Animal Cell Culture and Technology / Edition 2

by Michael Butler

Overview

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Product Details

ISBN-13:	9781859960493
Publisher:	Taylor & Francis
Publication date:	12/25/2003
Series:	THE BASICS (Garland Science)
Edition description:	Revised
Pages:	256
Product dimensions:	6.88(w) x 9.69(h) x (d)

Table of Contents

1. Introduction: The Use of Animal Cell Culture
2. Characteristics of Cells in Culture
3. Basic Equipment and Laboratory Design: What You need to Get Started in Cell Culture
4. Growth and Maintenance of Cells in Culture
5. Cell Line and Culture Monitoring
6. Genetic Engineering of Animal Cells in Culture
7. The Glycosylation of Proteins in Cell Culture
8. Hybridomas - Sources of Antibodies
9. Scaling up Animal Cell Culture
10. Modes of Culture for High Cell Densities
11. Production from Cell Culture
12. Mammalian Cell Products: Established and Potential

Editorial Reviews

Reviewer: Alla Arzumanyan, Ph.D.(Thomas Jefferson University)
Description: Cell culture has expanded enormously with new applications appearing all the time. This second edition offers a complete training manual with information on fundamental principles and new techniques of animal cell culture. This book's 12 chapters cover the essential skills and knowledge required to grow and maintain animal cells in vitro. Specific topics include genetic engineering of animal cells in culture, glycosylation engineering as a tool for optimizing pharmaceuticals, viral vaccines, and the use of monoclonal antibodies in human clinical applications. It also highlights the potential value of mammalian cell-derived products, as well as the risks associated with various cell culture products.
Purpose: The goal is to provide a grasp of basic concepts to enable researchers to move quickly to specialized work in the field. The book is a perfect guide for those developing their knowledge of cell culture techniques and/or those looking for a concise reference book.
Audience: According to the author, this book is written for a general audience interested in the field of animal cell culture and technology. This book will be particularly helpful for undergraduates, graduate students, technicians, and experienced industrial researchers who want to learn laboratory techniques for the study of animal cells.
Features: The book provides essential background information in parallel with easy to follow, step-by-step protocols for manipulating cells in culture. Each chapter includes an introduction, conclusion, references, and sources for further reading. Many line drawings illustrate key points. An excellent feature of this book is that the protocols are accompanied by basic theory with straightforward explanations and suggestions. The latter will give the students the opportunity to think critically while carrying out experiments. The updated edition offers links to other relevant publications. The author has a special interest in protein and lipid glycosylation.
Assessment: This book is written primarily as an introduction to cell culture research, providing background information, theory, and straightforward step-by-step laboratory protocols. It covers both classical and state-of-the-art methods that are used in the laboratory. The discussion of cutting edge techniques such as serum-free medium and "humanizing" monoclonal antibodies will help make this book a valuable resource for all laboratories involved in animal cell culture. It will be appreciated by a wide audience of scientists in the biomedical and life sciences.

Doody's Review Service

Reviewer: Alla Arzumanyan, Ph.D.(Thomas Jefferson University)
Description: Cell culture has expanded enormously with new applications appearing all the time. This second edition offers a complete training manual with information on fundamental principles and new techniques of animal cell culture. This book's 12 chapters cover the essential skills and knowledge required to grow and maintain animal cells in vitro. Specific topics include genetic engineering of animal cells in culture, glycosylation engineering as a tool for optimizing pharmaceuticals, viral vaccines, and the use of monoclonal antibodies in human clinical applications. It also highlights the potential value of mammalian cell-derived products, as well as the risks associated with various cell culture products.
Purpose: The goal is to provide a grasp of basic concepts to enable researchers to move quickly to specialized work in the field. The book is a perfect guide for those developing their knowledge of cell culture techniques and/or those looking for a concise reference book.
Audience: According to the author, this book is written for a general audience interested in the field of animal cell culture and technology. This book will be particularly helpful for undergraduates, graduate students, technicians, and experienced industrial researchers who want to learn laboratory techniques for the study of animal cells.
Features: The book provides essential background information in parallel with easy to follow, step-by-step protocols for manipulating cells in culture. Each chapter includes an introduction, conclusion, references, and sources for further reading. Many line drawings illustrate key points. An excellent feature of this book is that the protocols are accompanied by basic theory with straightforward explanations and suggestions. The latter will give the students the opportunity to think critically while carrying out experiments. The updated edition offers links to other relevant publications. The author has a special interest in protein and lipid glycosylation.
Assessment: This book is written primarily as an introduction to cell culture research, providing background information, theory, and straightforward step-by-step laboratory protocols. It covers both classical and state-of-the-art methods that are used in the laboratory. The discussion of cutting edge techniques such as serum-free medium and "humanizing" monoclonal antibodies will help make this book a valuable resource for all laboratories involved in animal cell culture. It will be appreciated by a wide audience of scientists in the biomedical and life sciences.

"...A valuable resource for all laboratories involved in animal cell culture. It will be appreciated by a wide audience of scientists in the biomedical and life sciences."
—March 2005

Doody Review Service

"... An excellent resource for undergraduate and graduate biology students as well as beginning scientific researchers."
—vol 7, no 12, 2004

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