Animal Cell Culture and Technology

Animal Cell Culture and Technology

by M. Butler
Animal Cell Culture and Technology

Animal Cell Culture and Technology

by M. Butler

eBookREV (REV)

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Overview

The Basics series provides the ideal introduction to new techniques in the laboratory. This series of books will allow you to use the techniques described, and to manipulate them to suit your own basic requirements.

This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for scientists intending to initiate some work in this field.

New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock (teleomeres) of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use.


Product Details

ISBN-13: 9780203427835
Publisher: Taylor & Francis, Inc.
Publication date: 12/25/2003
Series: The BASICS (Garland Science) Series
Sold by: Barnes & Noble
Format: eBook
File size: 6 MB

Table of Contents

1. The Development of Animal Cell Technology 2. Characteristics of Cells in Culture Protocols For Deriving Primary Cell Culture. Selecting and Cloning Cells 3. Growth and Maintenance of Cells in Culture Protocols For Sub-Culturing a Cell Line. Establishing a Stationary Culture. Establishing a Stirred Microcarrier Culture. Adapting Cells to Serum-Free Formulations 4. Cell Line and Culture Monitoring Protocols for Cell Counting. Karyotyping. Isozyme Analysis 5. Genetic Engineering of Animal Cells Protocols for Transfecting a Cell Line. Expressing a Protein 6. Hybridoma Technology Protocols for Cell Fusion. Producing Monoclonal Antibodies 7. Glycosylation of Proteins Protocols for Analysing Glycans Attached to Proteins 8. Bioreactors For Cell Culture Protocols for Scale-Up in a Stirred-Tank Bioreactor 9. Modes of Cell Culture Protocol for Establishing a Continuous Culture 10. Production of Biologicals from Cell Cultures.
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