Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models / Edition 2

by William S. M. Wold, Ann E. Tollefson

Overview

Adenovirus Methods and Prools, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the prools presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the prools in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

Product Details

ISBN-13:	9781617376696
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Medicine , #130
Edition description:	Softcover reprint of hardcover 2nd ed. 2007
Pages:	242
Product dimensions:	5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Manipulation of Early Region 4.- Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants.- Construction of Adenovirus Type 5 Early Region 1 and 4 Virus Mutants.- Construction of Mouse Adenovirus Type 1 Mutants.- Generation of Recombinant Adenovirus Using the Escherichia coli BJ5183 Recombination System.- Production and Release Testing of Ovine Atadenovirus Vectors.- Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3.- Adenovirus Capsid Chimeras.- Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis.- Evaluating Apoptosis in Tumor Cells Infected With Adenovirus Expressing p53 Tumor Suppressor Gene.- Growth and Purification of Enteric Adenovirus Type 40.- Immunocompetent, Semi-Permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses.- Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors.- A Real-Time PCR Method to Rapidly Titer Adenovirus Sks.- Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR.- Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins.- Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs.- Preparation and Titration of CsCl-Banded Adenovirus Sks.

Editorial Reviews

"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars

Reviews of the First edition:

"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today

". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters

From the Publisher

Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: As the title implies, this book provides details on the procedures that can be used to construct adenoviruses to be used as vectors. Each chapter specifically details laboratory procedures that can be used for these purposes.
Purpose: The book is intended to provide a "detailed description" of the methods used to grow, detect, and analyze adenoviruses. The overall purpose of growing and detecting these viruses is so that they can be used as vectors to transfer genes into cells or larger animals. This is of great interest now, since this could potentially provide a means of delivering genetic material as a drug.
Audience: Scientists working in the area of virology and gene transfer are the intended audience, but the book would be easy reading for most levels from the undergraduate student to the professional scientist. All the authors clearly have personal experience in using these techniques.
Features: The 18 chapters cover primarily the procedures used to grow adenoviruses and genetically manipulate the virus as well as the use of animal models to test the manipulated viruses. Each chapter starts with a summary and an introductory section, followed by a section on materials needed to perform the experimental protocols and then by specific methods to accomplish the experiments using adenoviruses. These sections are followed by notes on the procedures and are linked to the procedure by a number. Overall there is a great deal of information on laboratory technique. Some authors list very similar techniques, which can be confusing for readers trying to make decisions on protocols. It was difficult to continually go back to the notes section and then find where you left the procedure. It would help to place the notes close to where the reference is in the protocol. Also, some of the techniques seem dated and might be hard to perform in a laboratory just starting in this area. Overall, though, the book provides some good advice on using and growing adenovirus vectors.
Assessment: This book would be useful for scientists in this area. A change in the size and format of the book would help. It does not stay open very well and at a lab bench, this could be annoying. It would also be useful to have the hints and notes embedded into the methods, making it clearer for the user.

Doody's Review Service