Yeast Metabolic Engineering: Methods and Protocols

by Valeria Mapelli (Editor), Maurizio Bettiga (Editor)

Overview

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation prools for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, andtips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Prools, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Product Details

ISBN-13:	9781071624012
Publisher:	Springer US
Publication date:	07/04/2022
Series:	Methods in Molecular Biology , #2513
Edition description:	2nd ed. 2022
Pages:	295
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae.- Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae.- Tools for Genetic Engineering of the Yeast Hansenula polymorpha.- Molecular Tools and Prools for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory.- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.- Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains.- Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries.- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology.- Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach.- Evolutionary Engineering of Yeast.- Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains.- Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis.- ¹³C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case.- Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis.- QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast.- Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction and Other Applications.- Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae.- Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace.