Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols

by Alejandro Brun

Overview

This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered as well as an updated view of most options available for vaccine development, including the data obtained through experimental trials which contributes to the exploration and understanding of the immune mechanisms and immune correlates relevant in protection among different animal species. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Vaccine Technologies for Veterinary Viral Diseases: Methods and Prools facilitates access to well-established prools to those beginning in this interesting and laborious field as well as providing important basic knowledge when attempting a novel vaccine design or platform.

Product Details

ISBN-13:	9781493950003
Publisher:	Springer New York
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #1349
Edition description:	Softcover reprint of the original 1st ed. 2016
Pages:	265
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Vaccines and Vaccination for Veterinary Viral Diseases: A General Overview.- Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination.- Plant-Based Vaccine Antigen Production.- DNA Vaccines: Experiences in the Swine Model.- Novel Adjuvants and Immunomodulators for Veterinary Vaccines.- Polymerase Mechanism-Based Method of Viral Attenuation.- BacMam Platform for Vaccine Antigen Delivery.- Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines.- Generation of Recombinant Modified Vaccinia Virus Ankara Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus.- Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies.- Recombinant Swinepox Virus for Veterinary Vaccine Development.- Generation and Selection of Orf Virus (ORFV) Recombinants.- Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development.- Construction and Application of Newcastle Disease Virus-Based Vector Vaccines.- Chimeric Pestivirus Experimental Vaccines.- Analysis of the Cellular Immune Responses to Vaccines.