Table of Contents
About the Author ix
Acknowledgements xi
Look-Up Guide to Feature Boxes by Theme xii
Glossary and Definitions xiv
Notes xxiv
Introduction xxvii
1 Our Eyes and the Microscope 1
2 Light 29
3 Basic Microscope Optics 55
4 Microscope Anatomy and Design 75
5 Ergonomics 91
6 Optical Aberrations of the Microscope 101
7 The Microscope Objective 127
8 Condensers and Eyepieces 161
9 Illumination in the Microscope 177
10 Diffraction and Image Formation in Microscopy 211
11 Contrast Generation and Enhancement 243
12 Reflected-Light Microscopy 289
13 Polarised-Light Microscopy: Part 1 – Theory 317
14 Polarised-Light Microscopy: Part 2 – Applied 347
15 Fluorescence Microscopy 383
16 Fluorophores and Fluorescent Proteins 405
17 Optical Sectioning and Confocal Microscopy 425
18 Operating the Confocal Microscope 447
19 Light-Sheet Microscopy 483
20 Bleed-Through and Spectral Unmixing 507
21 Deconvolution 523
22 Multi-Photon Microscopy 543
23 Total Internal Reflection Fluorescence Microscopy 561
24 FRAP and FRET 569
25 Colocalisation 587
26 Super-Resolution Fluorescence Microscopy 613
27 Choosing a Microscope Platform and Core Imaging Facilities 637
28 Biological Specimen Preparation 663
29 Materials Specimen Preparation 687
30 Recording the Image: Part 1 – Theory 707
31 Recording the Image: Part 2 – Applied 733
Appendices
1 Buying, and Tendering for, a Light Microscope 769
2 Troubleshooting Poor Image Quality 773
3 The Michel-Lévy Interference Colour Chart 775
4 Cleaning and Maintenance of the Light Microscope 779
5 Selected Suppliers 783
6 Historical Background 787
7 Timeline of Key Events 791
Index 799