Unbiased Stereology: A Concise Guide

by Peter R. Mouton

Overview

This update to Peter R. Mouton’s pioneering work provides bioscientists with the concepts needed in order to apply the principles and practices of unbiased stereology to research involving biological tissues.

Mouton starts with a brief explanation of the history and theory of the process before defining the terms, concepts, and tools of unbiased stereological procedures. He compares and contrasts the procedures with less-exacting approaches to quantitative analysis of biological structure using specific examples from biomedical literature. The book incorporates existing best practices with new methodologies, such as the Rare Event Protocol, while simplifying the dense, often difficult literature on the subject to show the utility and importance of unbiased stereology. This clear, insightful guide goes a step further than other books on this subject by demonstrating not only how to use unbiased stereology but also how to interpret and present the results.

Written by the official U.S. representative to the International Society for Stereology, this is the most complete, up-to-date resource on the science of unbiased stereology. Those new to bioscience research as well as experienced practitioners will find that Mouton’s explanations are the perfect companion for stereology courses and workshops.

Product Details

ISBN-13:	9780801899850
Publisher:	Johns Hopkins University Press
Publication date:	08/15/2011
Pages:	200
Product dimensions:	5.90(w) x 8.90(h) x 0.40(d)
Age Range:	18 Years

About the Author

Peter R. Mouton is the director and chief executive officer of the Stereology Resource Center, Inc., a professor of stereology at the University of South Florida School of Medicine, and the author of Principles and Practices of Unbiased Stereology: An Introduction for Bioscientists, also published by Johns Hopkins. Through stereology workshops and short courses in North America, Europe, and Australia, he has trained thousands of biomedical researchers in the application of unbiased stereology.

Table of Contents

Preface ix

1 Elias Coins a Word 1

2 Solid 3-D Objects 5

3 Regional Volume Estimation 9

4 Area Estimation by Point Counting 15

5 Probe-Object Intersections 22

6 Volume By Cavalieri Point Counting 26

7 Accuracy and Precision 33

8 From 2-D to 3-D 47

9 Surface Area and Length 62

10 Total Object Number 71

11 Rare Events 90

12 Local Size Estimators 96

13 Do More, Less Well 105

14 Uncertainty 120

15 Computerized Stereology Systems 133

16 A Survey of Tissue 142

17 Peer Review Considerations 147

Appendix: Conceptual Framework for Organic Stereology 153

Glossary 155

Bibliography 161

Index 171

What People are Saying About This

Arun M. Gokhale

An excellent textbook for practical applications of the theoretically rigorous methods of state-of-art unbiased stereology.

— Arun M. Gokhale, Georgia Institute of Technology

From the Publisher

An excellent textbook for practical applications of the theoretically rigorous methods of state-of-art unbiased stereology.
—Arun M. Gokhale, Georgia Institute of Technology