Ubiquitin-dependent Protein Degradation

by Mark Hochstrasser

Overview

Ubiquitination and Protein Stability - Part B, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Topics of note include chapters on Assays of SUMO protease function in mammalian cells, In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation, Methods to study proteasome regulatory particle assembly, Native mass spectrometry approaches to study the proteasome, Single-molecule methods to study the ubiquitin-proteasome system, Assays for the function of ubiquitin in the mammalian endocytic pathway, and much more.

Product Details

ISBN-13:	9780128186671
Publisher:	Elsevier Science
Publication date:	03/23/2019
Series:	Methods in Enzymology , #619
Pages:	384
Product dimensions:	6.00(w) x 9.00(h) x (d)

About the Author

Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology
Yale University, USA.

Table of Contents

1. Assays for protein retrotranslocation in ERAD Sonya Neal, Sascha H. Duttke and Randolph Y. Hampton 2. Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae Rahul S. Samant and Judith Frydman 3. Methodologies to monitor protein turnover at the inner nuclear membrane Pei-Ling Tsai, Chenguang Zhao and Christian Schlieker 4. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 Bayan Mashahreh, Yuval Reiss, Reuven Wiener and Tommer Ravid 5. Methods for genetic analysis of mammalian ER-associated degradation Dara E. Leto and Ron R. Kopito 6. A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies Néstor García-Rodríguez and Helle D. Ulrich 7. Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome Randi G. Reed and Robert J. Tomko Jr. 8. Assays for ubiquitin-like protein ligation and proteasome function in archaea Xian Fu, Zachary Adams and Julie Maupin-Furlow 9. Structural mass spectrometry approaches to study the 20S proteasome Gili Ben-Nissan, Shay Vimer, Mark Tarnavsky and Michal Sharon 10. Single-molecule methods for measuring ubiquitination and protein stability Jason Hon and Ying Lu 11. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation Srinivasan Muniyappan and Byung-Hoon Lee 12. Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway Evan L. Guiney, Lu Zhu, Richa Sardana, Scott D. Emr and Matthew G. Baile 13. Detection of ubiquitinated targets in mammalian and Drosophila models Elena Maspero, Valentina Fajner, Janine Weber and Simona Polo 14. Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans Alexander Springhorn and Thorsten Hoppe 15. Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells Victoria Cohen-Kaplan, Ido Livneh, Yong Tae Kwon and Aaron Ciechanover

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