Transplantation Immunology: Methods and Protocols / Edition 1

by Philip Hornick

Overview

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HLA typing, detecting HLA antibodies, and monitoring T-cell response, and examine more specialized methods utilizing proteomics, laser dissection microscopy, and real-time polymerase chain reaction. The area of tolerance induction and reprogramming of the immune system is also covered, along with a discussion of up-to-date methods of organ preservation, of today's optimal immunosuppressive drug regimens, as well as the difficulty of mimicking chronic rejection in experimental models. Introductory chapters provide a theoretical update on current practices in renal, liver, islet, and lung transplantation and on the pathways of antigen presentation and chronic rejection.

Product Details

ISBN-13:	9781617376320
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/01/2010
Series:	Methods in Molecular Biology , #333
Edition description:	Softcover reprint of hardcover 1st ed. 2006
Pages:	430
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Current Status of Renal Transplantation.- Current Status of Liver Transplantation.- Current Status of Clinical Islet Cell Transplantation.- Current Status of Lung Transplantation.- Chronic Rejection in the Heart.- Direct and Indirect Allorecognition.- HLA Typing and Its Influence on Organ Transplantation.- Strategies for Gene Transfer to Solid Organs.- Nonviral Vectors.- Detection and Clinical Relevance of Antibodies After Transplantation.- 11 Reprogramming the Immune System Using Antibodies.- In Vitro Assays for Immune Monitoring in Transplantation.- Proteomics and Laser Microdissection.- Real-Time Quantitative Polymerase Chain Reaction in Cardiac Transplant Research.- Organ Preservation.- Pharmacological Manipulation of the Rejection Response.- Experimental Models of Graft Arteriosclerosis.