Topics in Fluorescence Spectroscopy: Volume 4: Probe Design and Chemical Sensing / Edition 1

by Joseph R. Lakowicz

Overview

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Product Details

ISBN-13:	9780306447846
Publisher:	Springer US
Publication date:	11/30/1994
Series:	Topics in Fluorescence Spectroscopy , #4
Edition description:	1994
Pages:	502
Product dimensions:	6.10(w) x 9.25(h) x 0.24(d)

About the Author

Dr. J.R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the sole author of the widely used text, Principles of Fluorescence Spectroscopy, also published by Kluwer Academic/Plenum Publishers, now in its Second Edition.

Table of Contents

Emerging Biomedical Applications of Time-Resolved Fluorescence Spectroscopy.- Principles of Fluorescent Probe Design for Ion Recognition.- Fluorescent Chemosensors for Cations, Anions, and Neutral Analytes.- Design and Applications of Highly Luminescent Transition Metal Complexes.- Fluorescence Probes Based on Twisted Intramolecular Charge Transfer (TICT) States and Other Adiabatic Photoreactions.- Red and Near-Infrared Fluorometry.- Near-Infrared Fluorescence Probes.- Fluorescence Spectroscopy in Turbid Media and Tissues.- Real-Time Chemical Sensing Employing Luminescence Techniques.- Lifetime-Based Sensing.- Fiber Optic Fluorescence Thermometry.- Instrumentation for Red/Near-Infrared Fluorescence.- Application of Fluorescence Sensing to Bioreactors.- Principles of Fluorescence Immunoassay.

Editorial Reviews

From reviews of previous volumes:

‘The [editor] has mastered a commendable task; the topics appear to be well covered, and the volumes will enhance the collection of the fluorescence spectroscopist.'
Applied Spectroscopy

‘Indispensable for researchers in the life sciences, should also be consulted by analytical chemists.'
Trends in Analytical Chemistry

From the Publisher

Summarizes the principles required for biomedical applications of time-resolved fluorescence spectroscopy. Many of the chapters describe the development of red/NIR probes and the mechanisms by which analytes interact with the probes and produce spectral changes. Other chapters describe the unique opportunities of red/NIR fluorescence and the types of instruments suitable for such measurements. Also included is a description of the principles of chemical sensing based on lifetimes, and an overview of the important topic of immunoassays. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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