The Immunological Synapse Part A

by Elsevier Science

Overview

The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more.

Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L.

• Covers various methods related to the study of the immunological synapse
• Includes detailed, point-by-point, methods as well as various important notes
• Provides the authority and expertise from an international board of leading scientists

Product Details

ISBN-13:	9780323901550
Publisher:	Elsevier Science
Publication date:	01/16/2023
Series:	ISSN
Sold by:	Barnes & Noble
Format:	eBook
Pages:	206
File size:	18 MB
Note:	This product may take a few minutes to download.

About the Author

Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion.
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Table of Contents

1. Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators
   Marie Juzans, Celine Cuche, Vincenzo Di Bartolo, and Andres Alcover
2. Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse
   Sara Fernandez-Hermira, Irene Sanz-Fernandez, Marta Botas, Victor Calvo, and Manuel Izquierdo
3. P815-based redirected degranulation assay to study human NK cell effector functions
   Iñigo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego
4. Cytotoxic and chemotactic dynamics of NK cells quantified by live-cell imaging
   Yanting Zhu and Jue Shi
5. Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay
   Ondrej Cerny
6. Assessment of membrane lipid state at the natural killer cell immunological synapse
   Yu Li and Jordan S. Orange
7. Study of the effects of NK-tumor cell interaction by proteomic analysis and imaging
   Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi
8. Protocol for the murine antibody-dependent cellular phagocytosis assay
   Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto
9. Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers
   Melanie Grusdat, Catherine Dostert, and Dirk Brenner
10. An in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissue
    Nicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte Wieten
11. Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer
    Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu
12. Potency monitoring of CAR T cells
    Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown