Terminal Transferase in Immunobiology and Leukemia

by Umberto Bertazzoni (Editor)

Overview

This book contains the proceedings of a conference devoted to the study of the structure and function of Terminal deoxynucleotidyl Transferase (TdT) and its utilization as biochemical marker in immuno­ biology and leukemia, held in Elba, Italy on May 28-31, 1981. The en­ zyme has been known to nucleic acid biochemists for more than 20 years and has proved to be an excellent tool for making deoxypolymers, label­ ing DNA fragments, and adding homopolymer tails to restriction endonu­ clease fragments from DNA. Since the discovery of its peculiar tissue distribution, normally restricted to the thymus and bone marrow, and of its abnormal occur­ rence in human leukemic lymphoblasts, TdT has become one of the most widely used markers in the study of lymphocyte differentiation and in the classification of hematopoietic neoplasia. The subject seemed most appropriate for a meeting where molecular and cellular biologists, immunologists and hematologists could convene for the first time to discuss both basic research and the clinical aspects of the problem. Among the goals achieved by this workshop was the sharing of information about the enzymology of TdT, biochemical and immunological methodology and the correlation of TdT with other markers in the diag­ nosis of leukemia. The remarkable accordance of results, presented here by five independent hematological institutions, from analysis of TdT in several thousands of leukemic patients, marks the importance of this enzyme as a diagnostic and prognostic tool in these diseases.

Product Details

ISBN-13:	9781468489316
Publisher:	Springer US
Publication date:	06/15/2012
Series:	Advances in Experimental Medicine and Biology , #145
Edition description:	Softcover reprint of the original 1st ed. 1982
Pages:	393
Product dimensions:	7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

Section I Structures and Properties of Terminal Deoxynucleotidyl Transferase.- Terminal Transferase: Past to Present.- Purification of Terminal Deoxynucleotidyl Transferase from Pig Thymus: Identification of 42,000 and 57,000 Dalton Species.- Purification of Terminal Deoxynucleotidyl Transferase of 60,000 Dalton from Mannalian Thymus and Thymoma by Immunoadsorbent Column and Comparison of Peptide Structures.- Structure of Calf Thymus Terminal Deoxynucleotidyl Transferase.- Purification and Characterization of Multiple Forms of Terminal Transferase from Human Leukemic Cells.- Purification and Properties of Chick Terminal Deoxynucleotidyl Transferase (TdT).- Restriction Analysis of Cloned Heteropolymeric DNA Synthesized in vitro by TdT.- Terminal Deoxynucleotidyl Transferase Helps DNA Polymerase— Bypass Thymine Dimer.- Inhibitors of DNA Polymerases: Their Selectivity and Mode of Action.- Section II Development of Immunological Reagents for Detection of Terminal Deoxynucleotidyl Transferase.- Development of Antibodies and Immunological Methods for Terminal Transferase Assay..- Use of Immimoflurorescence Tests for TdT in Human Hematologic Malignancy.- Section III Use of Terminal Deoxynucleotidyl Transferase as a Marker for Studies on Differentiation of Lymphocytes.- Ontogeny of Terminal Deoxynucleotidyl Transferase Containing Lymphocytes in R ts Mice.- Effects of Biological Response Modifiers on the Growth and Differentiation of Terminal Deoxynucleotidyl Transferase Containing Lymphocytes.- Murine Thymocyte Subpopulations: Two and Three Parameter Analysis with Various Markers.- Terminal Transferase Positive Cells in the Human Bone Marrow and Thymus.- Disappearance of Nuclear TdT in RPMI-8402 Following TPA Treatment.- Terminal Deoxynucleotidyl Transferase in Human FetalTissues.- Effect of Nucleosides on Human T and B Lymphoblastoid Cell Lines.- 20'-Hydroxysteroid Dehydrogenase (20?SDH) - A New Enzymatic Marker for Pre-T and T Lymphocytes.- Hybrids Between Myeloma Cells and Thymocytes - An Attempt to Approach the Biological Function of Terminal Transferase.- Section IV Terminal Transferase as a Marker for Malignant Lymphoid Cells.- The Bollum Enzyme in Leukemia and Lymphoma Cells: the First Decade.- Prognostic Significance of Terminal Transferase Activity and Other Factors in Childhood Acute Lymphoblastic Leukemia.- The Prognostic Significance of Terminal Deoxynucleotidyl Transferase (TdT) in Patients with Leukemias and Malignant Lymphomas.- Clinical Relevance of Terminal Transferase and Adenosine Deaminase in Leukemia.- Studies of Terminal Deoxynucleotidyl Transferase in Normal and Neoplastic Human Cells.- Nuclear Terminal Deoxynucleotidyl Transferase (TdT) in Leukemic Infiltrates of Testicular Tissue.- Phenotypic Changes During Blast Crisis of CML Characterized by Occurrence and Loss of TdT.- Enrichment of Terminal Deoxynucleotidyl Transferase Activity by Cell Separation.- TdT-Positive and TdT Negative Human Leukemic Cells: Specific Density and Morphology.- Normal Mononuclear Blood Cells in Diffusion Chambers: Occurrence of cALLA and TdT.- Enzymatic Properties in Pericellular Membranes of Leukemia Cells.