Techniques in Protein Chemistry III

by Ruth Hogue Angeletti (Editor)

Overview

Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.

Product Details

ISBN-13:	9781483259468
Publisher:	Elsevier Science & Technology Books
Publication date:	06/28/2014
Sold by:	Barnes & Noble
Format:	eBook
Pages:	572
File size:	13 MB
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Table of Contents

ForewordPrefaceAcknowledgmentsSection I Protein Microsequence Methods Protein and Peptide Recovery from PVDF Membranes Automated C-Terminal Sequencing of Peptides Elution and Internal Amino Acid Sequencing of PVDF-Blotted Proteins Evaluation of Protein Sequencing Core Facilities: Design, Characterization, and Results from a Test Sample (ABRF-91SEQ) Optimization of Solid Phase Peptide Sequencing Evaluation of the Blott Cartridge for Enhanced Gas Phase Sequencing at Maximum Sensitivity Reusing PVDF Electroblotted Protein Samples After N-Terminal Sequencing to Obtain Unique Internal Amino Acid Sequence Chromatographic Carbon as an Inert Sample Adsorbent for Protein Sequencing Na-Acylaminoacyl-Peptide Hydrolase: Specificity and Use toUnblock N-Acetylated ProteinsSection II Protein Microsequencing Workshop Strategies for the Isolation of Peptides from Low-Abundance Proteins for Internal Sequence Analysis High-Sensitivity Peptide Mapping Utilizing Reversed-Phase Microbore and Microcolumn Liquid Chromatography Microscale Protein Isolation and Microsequencing Workshop: An OverviewSection II? Peptide/Protein Separation Techniques Micropreparative Separation of Tryptic Digests by Capillary Electrophoresis and Characterization by Protein Sequencing Estimation of Protein Isoelectric Points by Capillary Electrophoresis Using MICRO-COAT™ Capillary Electrophoresis for Preparation of Peptides and Direct Determination of Amino Acids Perfusion Chromatography: A Novel Tool for Protein Purification and Analysis Comparison of Coupling Procedures for Development of Affinity Membranes: Optimization of the CDI MethodSection IV Peptide Synthesis Randomization of Amino Acid Sequence: An Important Control in Synthetic Peptide Analogue Studies of Nucleic Acid Binding Domains Comparative Analysis of Synthetic Peptides by Free-Solution Capillary Electrophoresis (FSCE) and Strong-Cation Exchange (SCX)-HPLC Immunoreactive Synthetic Peptide Epitope Mapping with Structural Validation Using Electrospray Mass Spectrometry TFMSA/TFA Cleavage in t-Boc Peptide Synthesis Synergy of Semisynthesis and Site-Directed Mutagenesis: Manipulating Genes to Create Sites for Autocatalytic Protein Fragment Religation State-of-the-Art Peptide Synthesis: Comparative Characterization of a 16-mer Synthesized in 31 Different LaboratoriesSection V Amino Acid Analysis Applications of Automatic PTC Amino Acid Analysis LC-Determination of D- and L-Amino Acids in Peptide Hydrolysates by Pre-column Derivatization with a Chiral Reagent Amino Acid Analysis of PVDF Bound Proteins Collaborative Trial Analyses of ABRF-91AAASection VI Structural Analysis of Glycoproteins Identification of O-Glycosylation Sites with a Gas Phase Sequencer Glycoform, Glycotype Linkage, and Branching Detail of the CD2 Adhesion Domain by Desolvation Mass Spectrometry: SFCI-MS and ESI-MS Characterization of the Glycosylation on Recombinant Human Low-Affinity Nerve Growth Factor Receptor Nonenzymic Glycation of Hemoglobin: Structural Determinants of Site Selectivity A Unified Approach to Glycoprotein Primary Structure Analysis: Identification, Isolation, and Characterization of Both Peptide and Pendant Carbohydrate of GlycopeptidesSection VII Protein Folding and Conformation Refolding of Human Recombinant Insulin-like Growth Factor II (IGF-II) in Vitro, Using a Solubilizing Affinity Handle Conformational Stability of the Molten Globule of Cytochrome C: Role of Electrostatic Repulsion Calcium-Induced Folding of Troponin-C: Formation of Homodimeric and Heterodimeric Two-Site Domains from Synthetic Peptides The Role of Aqueous Solvation in Protein Folding: Insights from Aqueous-Alkane Partitioning Molecular Characterization of an Aggregate Formed by a Bovine Growth Hormone Folding Intermediate Use of Sequence Hydrophobic Moment to Analyze Membrane Interacting Domai