Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers

by Syed A. Aziz, Rekha Mehta

Overview

This authoritative volume examines immunohishemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with a comprehensive review of the methods and approaches for immunohishemical staining in various target tissues. It explores the tissue-antigen and antibody-specific problems that may be encountered during the staining procedures and provide potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents, monkeys and aquatic animal tissues.

Product Details

ISBN-13:	9781493979653
Publisher:	Springer New York
Publication date:	03/27/2019
Edition description:	Softcover reprint of the original 1st ed. 2016
Pages:	231
Product dimensions:	6.10(w) x 9.25(h) x (d)

About the Author

Syed A Aziz, Ph.D.

Regulatory Toxicology Research Division, Bureau of Chemical Safety

Food Directorate, Health Products and Food Branch

Health Canada

Adjunct professor, Faculty of Medicine Dept. of Pathology

University of Ottawa

Rekha Mehta, Ph.D.

Regulatory Toxicology Research Division, Bureau of Chemical Safety

Food Directorate, Health Products and Food Branch

Health Canada.

Table of Contents

1.Immunohishemistry – Overview, Its Potential and Challenges.- 2.Tissue Micro Array (TMA) Technology and its Potential Applications in Toxicology and Toxicological Immunohishemistry.- 3.Importance of Fluorescent In Situ Hybridization (FISH) in Rodent Tumors.- 4.Quantitation of Immunohishemistry by Image Analysis Technique.- 5.Immunohishemistry in the Study of Cancer Biomarkers for Oncology Drug Development.- 6.Male Reproductive Toxicology and the Role of Immunohishemistry.- 7. Immunohishemistry and Female Reproductive Toxicology: The Ovary and Mammary Gland.- 8.Immunohishemistry on Rodent Circulatory System: Its Possible Use in Investigating Hypertension.- 9.Role of Lymphoid/Mucosa-Associated Lymphoid Tissue Markers in Toxicological Immunohishemistry. 10.Bone Markers and Immunohishemistry Changes in Toxic Environments.- 11. Biomarkers of Skeletal Muscle Injury in the Rat.