Single-Channel Recording

by Bert Sakmann (Editor), Erwin Neher (Editor)

Overview

Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

Product Details

ISBN-13:	9781441912305
Publisher:	Springer US
Publication date:	12/09/2009
Edition description:	2nd ed. 1995
Pages:	700
Product dimensions:	7.10(w) x 10.00(h) x 1.80(d)

Table of Contents

I. Methods.- 1 Electronic Design of the Patch Clamp.- 2 Geometric Parameters of Pipettes and Membrane Patches.- 3 Science and Technology of Patch-Recording Electrodes.- 4 Enzymatic Dispersion of Heart and Other Tissues.- 5 A Primer in Cell Culture for Patchologists.- 6 Patch-Clamped Liposomes: Recording Reconstituted Ion Channels.- 7 Tight-Seal Whole-Cell Recording.- 8 The Loose Patch Clamp.- II. Concepts and Analysis.- 9 The Principles of the Shastic Interpretation of Ion-Channel Mechanisms.- 10 Conformational Transitions of Ionic Channels.- 11 Fitting and Statistical Analysis of Single-Channel Records.- 12 Automated Analysis of Single-Channel Records.- 13 Analysis of Nonstationary Channel Kinetics.- 14 An Example of Analysis.- 15 Membrane Current and Membrane Potential from Single-Channel Kinetics.- III. Patch Clamp Data.- 16 Bursts of Openings in Transmitter-Activated Ion Channels.- 17 Is the Acetylcholine Receptor a Unit-Conductance Channel?.- 18 Analysis of Single-Channel Data from Glutamate Receptor-Channel Complexes on Locust Muscle.- 19 Experimental Approaches Used to Examine Single Glutamate-Receptor Ion Channels in Locust Muscle Fibers.- 20 Cholinergic Chloride Channels in Snail Neurons.- 21 Single-Channel Analysis in Aplysia Neurons: A Specific K + Channel is Modulated by Serotonin and Cyclic AMP.- 22 Cholecystokinin and Acetylcholine Activation of Single-Channel Currents via Second Messenger in Pancreatic Acinar Cells.- 23 Observations on Single Calcium Channels: An Overview.- 24 Potassium and Chloride Channels in Red Blood Cells.- 25 The Influence of Membrane Isolation on Single Acetylcholine-Channel Current in Rat Myotubes.

Editorial Reviews

From reviews of the First Edition:

"A landmark in the field of membrane biophysics." (Science)

"A first-class laboratory guide...an indispensable reference." (Neuroscience)

From the Publisher

The single-channel recording technique has reached the status of a routine method, and the view that conductance changes in biological membranes are caused by the openings and closings of ion channels is now almost universally accepted. This volume contains 22 chapters in four parts: introduction to patch clamping; methods; analysis; and "classics." There is also an appendix on improved patch-clamp techniques for high-resolution current recording from cells and cell- free membrane patches. The first edition was published in 1983. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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