Serum/Plasma Proteomics: Methods and Protocols

by David W. Greening (Editor), Richard J. Simpson (Editor)

Overview

Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Prools, Second Edition is a comprehensive resource of prools for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed prools for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Serum/Plasma Proteomics: Methods and Prools, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.

Product Details

ISBN-13:	9781071629772
Publisher:	Springer US
Publication date:	02/14/2023
Series:	Methods in Molecular Biology , #2628
Edition description:	3rd ed. 2023
Pages:	596
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Prools for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion.- Collection of plasma samples in areas with limited healthcare access.- Blood Collection Processing & Handling for Plasma and Serum Proteomics.- Preparation of cryoprecipitate and cryo-depleted plasma for proteomic research analysis.- High throughput proteome profiling of plasma and native plasma complexes using native chromatography.- High-throughput and in-depth proteomic profiling of 5 µL plasma and serum using TMTpro 16plex.- In depth data-independent acquisition proteomics of human plasma identifies low abundant proteins.- In-depth Blood Proteome Profiling by Extensive Fractionation and multiplexed quantitative mass spectrometry.- Early cancer biomarker discovery using DIA-MS proteomic analysis of EVs from peripheral blood.- Strategies to enrich, identify and characterize glycoproteome in blood plasma using Liquid Chromatography High Resolution Mass Spectrometry.- Proteome analysis of whole blood collected by volumetric absorptive microsampling.- Proteomic applications and considerations: from research to patient care.- Immunoaffinity mass spectrometry diagnostic tests for multi-biomarker assays.- Secretome profile of leukocyte-platelet rich fibrin (L-PRF) membranes.- Quantification of proteins in blood by absorptive microtiter plate-based affinity purification coupled to liquid chromatography-mass spectrometry.- Glycomics-assisted Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for analysis of low abundance peptide hormones in plasma.- In-depth proteomic analysis of blood circulating small extracellular vesicles.- Prool for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-based Proteomic Identification.- Analysis of ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using Multiple Reaction Monitoring.- Generation of red blood cell nanovesicles as a delivery tool.- Progress in targeted mass spectrometry (parallel accumulation-serial fragmentation) and its application in plasma/serum proteomics.- Offline peptide fractionation and parallel reaction monitoring MS for the quantitation of low-abundance plasma proteins.- Profiling Serum Intact N-Glycopeptides Using Data-Independent Acquisition Mass Spectrometry.- Semi-automated lectin magnetic bead array (LeMBA) for translational serum glycoprotein biomarker discovery and validation.- Accessing antibody-reactivities in serum or plasma to (auto-)antigens using multiplexed bead-based protein immunoassays.- Absolute quantitative targeted proteomics assays for plasma proteins.- Rapid and quantitative enrichment of peptides from plasma for mass spectrometric analysis.- Comprehensive targeted lipidomic profilingfor research and clinical applications.- Multiplexed bead-based peptide immunoassays for the detection of antibody reactivities.- Array-based multiplex and high-throughput serology assays.- Bioinformatic tools and knowledgebases to assist generating targeted assays for plasma proteomics.- Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection.