Selected Topics in Mass Spectrometry in the Biomolecular Sciences

by Richard M. Caprioli (Editor), A. Malorni (Editor), G. Sindona (Editor)

Overview

Many fundamental aspects of the methods used in mass spectrometry are here presented by outstanding scientists, with reference to very recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are presented, together with optimised GC/MS interfacing systems and tools for quantitative analysis. A comprehensive treatment of modern instrumentation for mass analysis and detection is also included. The major part of the book deals with bioanalytical applications to peptides, proteins, oligonucleotides, polysaccharides, lipids and plant metabolites. Several papers are devoted to the evaluation of adduct formation between DNA and carcinogens. Environmental applications are also included, with examples of some specific cases.
Fundamentals and applications are treated with the same degree of depth: the first two parts of the book therefore provide a basis for the understanding of the biomolecular applications section.
Audience: Ideal for advanced graduate students of chemistry who have learned some basic mass spectrometry. Also useful for Ph.D. students in chemistry, biology and medicine. Of value to researchers in academic and industrial laboratories.

Product Details

ISBN-13:	9780792348498
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/31/1997
Series:	NATO Science Series C: (closed) Series , #504
Pages:	612
Product dimensions:	6.69(w) x 9.61(h) x 1.31(d)

Table of Contents

	Preface	IX
	Contributing Authors	XI
	Participants	XVII
Part I	Methods in Mass Spectrometry
	Electrospray Ionization: Theory and Application	3
	Atmospheric Pressure Ionization (ESI and APCI) Theory and Application	17
	Matrix-Assisted Laser Desorpition-Ionization (MALDI) Mass Spectrometry: Principles and Applications	33
	High Sensitivity ESI/MS and MALDI MS	55
	Optimised GC/MS Interfacing Systems and Operating Conditions	85
	Quantitative Analysis by Mass Spectrometry: Some Important Considerations	103
	Optimization of Mass Spectrometric Ionisation Efficiency Data	121
	Role of Internal Energy in Mass Spectrometric Fragmentation	129
	Internation of Metal Ions and Protons with Simple Biomolecules. Structure Information from Bond Energies and Entropy Effects	143
	A Promising Marriage Between Theory and Experiment: Density Functional Method Versus Mass Spectrometry	163
Part II	Instrumentation for Mass Analysis and Detection
	Recent Advances in Magnet Sector Mass Spectrometry	183
	Quadrupole Mass Filters and Quadrupole Ion Traps	213
	Time-of-Flight Mass Spectrometry	239
	Combined Analyzer Technologies	263
	Fourier Transform Mass Spectrometry	287
Part III	Application to Biomolecules
	Protein Characterization by MS in Pharmaceutical Industry. Structural Characterization of Modified Forms of Recombinant Hirudin Sequence Variant 1 and Direct Batch Analysis	317
	Folding Pathways of Disulphide Containing Proteins	347
	Integration of Chemical Modification Reactions and Mass Spectrometric Methods in Protein Characterisation	361
	PSD-MALDI Analysis of Peptides	377
	Development of a Mass Spectrometric Approach for the Characterisation of Hemoglobin Adducts	399
	Reactivity of Antineoplastic Drugs with Model Peptides Studied by Advanced Mass Spectrometry Methodologies	413
	Applications of Mass Spectrometry in Biochemical Studies of Nucleosides, Nucleotides and Nucleic Acids	427
	MALDI Mass Analysis of Oligonucleotides	455
	DNA Adducts in Human Carcinogenesis. Clues to Etiology and Mechanism	465
	MALDI MS of Oligo and Polysaccharides	477
	Mass Spectrometry of Isoeicosanoids as Novel Lipid Substances	501
	The Role of Mass Spectrometry in the Characterization of Plant Metabolites from Crude Extracts	517
	Environmental Applications of Mass Spectrometry: Toxaphene Analysis	533
	The Determination of Polar Compounds in the Aquatic Environment	559
	Some Mass Spectrometry Contributions in the Study of Cultural Heritage	575
	Index	581

Editorial Reviews

The proceedings from the July 1996 symposium include sections on methods in mass spectrometry; instrumentation for mass analysis and detection; and application to biomolecules. Topics include ionization methods such as electrospray and MALDI techniques which are currently under development and refinement; GC/MS and quantitative analysis; the impact of mass spectrometric methods in protein analysis on pharmaceutical industry; the possibility of obtaining structural information from intact proteins with reference to their secondary structure as well; and the contribution of the methodology to cancer therapy. Annotation c. by Book News, Inc., Portland, Or.

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