Regulatory Protein Modification: Techniques and Protocols / Edition 1

by Hugh C. Hemmings

Overview

Hugh Hemmings' Regulatory Protein Modification: Techniques and Prools offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also

offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today.

Regulatory Protein Modification: Techniques and Prools will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.

Product Details

ISBN-13:	9781617370304
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Neuromethods , #30
Edition description:	Softcover reprint of hardcover 1st ed. 1997
Pages:	446
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

About the Author

Hemmings, Hugh C., Jr., MD, PhD (Cornell Univ)

The contributors represent the specialties of neuroscience, biochemistry, molecular biology, and neurobiology. Most are from universities in the U.S., the U.K., and Norway, including Rockefeller Univ, Univ of Leeds, Univ of Oslo, and Oregon State Univ.

Table of Contents

Analysis of Protein Phosphorylation in Intact Cells and Extracts.- Analysis of Protein Dephosphorylation in Intact Cells and Extracts.- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes).- Protein Kinase and Phosphatase Inhibitors.- Phosphorylation State-Specific Antibodies.- Protein Tyrosine Phosphorylation.- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis.- Protein methylation in the nervous system.- Long-Chain Fatty Acylation of Proteins.- Protein ADP-Ribosylation.- Glycosylation and Glycosylphosphatidylinositol Membrane Anchors.