Real-Time PCR

by Edited by M. Tevfik Dorak

Overview

Real-Time PCR provides the basic principles of applied real-time PCR and provide a firm grounding for those who wish to develop further applications.

Product Details

ISBN-13:	9780203967317
Publisher:	Taylor & Francis, Inc.
Publication date:	02/08/2007
Sold by:	Barnes & Noble
Format:	eBook
Pages:	333
File size:	3 MB

About the Author

Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK

Table of Contents

1. An Introduction to Real-time PCR 2. Data Analysis and Reporting 3. Relative Quantification 4. Normalisation 5. High-Throughput Primer and Probe Design 6. Quantitative Analysis of Ocular Gene Expression 7. Quantitative Gene Expression by Real-time PCR 8. Real-time PCR Using SYBR Green 9. High-Resolution Melting Analysis for Scanning and Genotyping 10. Quantitative Analyses of DNA Methylation 11. Mitochondrial DNA Analysis 12. Real-time Immuno-PCR 13. Clinical Microbiology 14. Clinical Virology 15. Solid Organ Transplant Monitoring 16. Real-time PCR Applications in Haematology 17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies