Proteome Research: Mass Spectrometry / Edition 1

by Peter James

Overview

Recent advances in large-scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale idenfification of these proteomes and their modifications. An understanding of these methods is critical for scientists in the "Post-Genome" era.

Product Details

ISBN-13:	9783540672562
Publisher:	Springer Berlin Heidelberg
Publication date:	12/12/2000
Series:	Principles and Practice
Edition description:	Softcover reprint of the original 1st ed. 2001
Pages:	274
Product dimensions:	6.10(w) x 9.25(h) x 0.02(d)

About the Author

A former film producer, British author Peter James is the bestselling mystery author of the Detective Superintendent Roy Grace series as well as other thriller novels. Over 15 million copies of his books are in print and he has won many awards for his writing such as the 2016 CWA Diamond Dagger for sustained excellence. James’s mother has the distinction of being the former glovemaker to Queen Elizabeth II. He lives in England.

Table of Contents

1 Mass Spectrometry and the Proteome.- 2 Basics of Triple-Stage Quadrupole/Ion-Trap Mass Spectrometry: Precursor, Product and Neutral-Loss Scanning. Electrospray Ionisation and Nanospray Ionisation.- 3 The Basics of Matrix-Assisted Laser Desorption, Ionisation Time-of-Flight Mass Spectrometry and Post-Source Decay Analysis.- 4 Data-Controlled Micro-Scale Liquid Chromatography — Tandem Mass Spectrometry of Peptides and Proteins: Strategies for Improved Sensitivity, Efficiency and Effectiveness.- 5 Solid-Phase Extraction-Capillary Zone Electrophoresis-Mass Spectrometry Analysis of Low-Abundance Proteins.- 6 Protein Identification by Peptide-Mass Fingerprinting.- 7 Protein Identification by SEQUEST.- 8 Interpreting Peptide Tandem Mass-Spectrometry Fragmentation Spectra.- 9 Automated Interpretation of Peptide Tandem Mass Spectra and Homology Searching.- 10 Specific Detection and Analysis of Phosphorylated Peptides by Mass Spectrometry.- 11 Glycoproteomics: High-Throughput Sequencing of Oligosaccharide Modifications to Proteins.- 12 Proteomics Databases.- 13 Quo Vadis.