Table of Contents
Foreword Sam Hanash iii
Preface v
Contributors xi
1 Protein Biochips and Array-Based Proteomics Ian Humphery-Smith 1
2 Ultrasensitive Microarray-Based Ligand Assay Technology Roger Ekins Frederick Chu 81
3 Practical Approaches to Protein Microarrays Brian Haab 127
4 Protein Biochips: Powerful New Tools to Unravel the Complexity of Proteomics? Steffen Nock Peter Wagner 145
5 Functionalized Surfaces for Protein Microarrays: State of the Art, Challenges, and Perspectives Erik Wischerhoff 159
6 High-Throughput Protein Expression, Purification, and Characterization Technologies Stefan R. Schmidt 173
7 Miniaturized Protein Production for Proteomics Michele Gilbert Todd C. Edwards Christa Prange Mike Malfatti Ian R. McConnell Joanna S. Albala 203
8 Protein Profiling: Proteomes and Subproteomes Eric T. Fung Enrique A. Dalmasso 217
9 Shotgun Proteomics and Its Applications to the Yeast Proteome Anita Saraf John R. Yates III 233
10 Forward and Reverse Proteomics: It Takes Two (or More) to Tango David E. Hill Nicolas Bertin Marc Vidal 255
11 Dynamic Visualization of Expressed Gene Networks Ingrid Remy Stephen W. Michnick 277
12 High-Throughput Structural Biology and Proteomics Wuxian Shi David A. Ostrov Sue Ellen Gerchman Jadwiga H. Kycia F. William Studier William Edstrom Anne Bresnick Joel Ehrlich John S. Blanchard Steven C. Almo Mark R. Chance 299
13 Integration of Proteomic, Genechip, and DNA Sequence Data Leah B. Shaw Vassily Hatzimanikatis Amit Mehra Kelvin H. Lee 325
14 The Proteomics Market Steven Bodovitz Julianne Dunphy Felicia M. Gentile 337
Index 351
