Principles of Genomics and Proteomics

by Rakeeb Ahmad Mir, Sheikh Mansoor Shafi PhD, Sajad Majeed Zargar Ph.D.

Overview

Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction.

A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments.

• Provides a comprehensive accounting of the technical aspects of genomic and proteomic analysis and applications in living organisms
• Includes detailed and standardized coverage of basic research methods in areas covered
• Presents advanced applications of genomics and proteomics that are highlighted with major significance in medical and agricultural fields

Product Details

ISBN-13:	9780323993784
Publisher:	Elsevier Science
Publication date:	01/25/2023
Sold by:	Barnes & Noble
Format:	eBook
Pages:	274
File size:	42 MB
Note:	This product may take a few minutes to download.

About the Author

Rakeeb Ahmad Mir is Assistant Professor, Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Science Campus, Nunner, Ganderrbal, Srinagar, Jammu&Kashmir, India. He is having 6 years of Research and Teaching Experience in different subjects of Biotechnology. His research interests include Molecular taxonomy, Nematode genomics and basic research in proteomics. In addition, he is deeply involved in teaching courses such as, Cell Biology, Signal transduction and Cancer Biology, Molecular Biology, Genomics and Proteomics and Nematode genomics at University level. Dr Rakeeb has published various research and review papers, book chapters on Molecular taxonomy of nematodes, Proteomic techniques and stress tolerant crop plants. Since he has considerable experience in the related fields pertaining to the title of this book.
Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Dr. Mansoor has been dedicated to contributing significantly to his field and has displayed a strong commitment to becoming a highly accomplished scientist. His expertise spans various areas, including biochemistry, molecular biology, and genetics. Dr. Mansoor's accomplishments are notable, including over more than 50 research and review papers published in esteemed journals such as Frontiers, Plos One, Scientific Reports, Journal of Fungi (JoF), Planta, Chemosphere, JEMA and Molecules etc. He has also authored 5 books and more than ten book chapters, and holds credit for filing two patent applications. He has showcased his knowledge through guest lectures on topics like sequence assembly, alignment, submission tools, and phylogenetic analysis. He has received practical training in advanced techniques in modern biology and related fields. Dr. Mansoor has been recognized with numerous national and international accolades, including prestigious awards such as Best Thesis, Young Scientist, and recognition for his exceptional oral and poster presentations. He holds membership in a significant big grant (BIRAC) awarded by the Department of Biotechnology (DBT) in India. In addition to his accomplishments, Dr. Mansoor plays an active role as a Review Editor for Frontiers in Ethnopharmacology, and he also contributes as a reviewer for esteemed journals like 3 Biotech, Cell Reports, Frontiers, Plos One, and JARMAP. His unwavering dedication, profound expertise, and active involvement underscore his unwavering commitment to advancing scientific knowledge in his field.
Sajad Majeed Zargar, Ph.D, is currently an Assistant Professor at Sher-e-Kashmir University of Sciences&Technology in India and visiting Professor at University of Padova, Italy. Dr. Zargar is the recipient of the CREST overseas fellowship from DBT, the Goho grant from Govt. of Japan, and the Erasmus Fellowship from European Union. He is also a member and representative of INPPO (International Plant Proteomics Organisation). His editorial activities and scientific memberships include publishing research and review articles in international journals and is also reviewer.

Table of Contents

1. Introduction to genomics and proteomics
2. Genome mapping
3. Analysis of genomes-I
4. Analysis of genomes-II
5. Nutrigenomics
6. Analysis of proteomes-I
7. Analysis of proteomes-II
8. Analysis of proteomes-III
9. Analysis of proteomes-IV
10. Beyond genomics and proteomics

What People are Saying About This

From the Publisher

A complete and detailed practical guide to Genomic and Proteomic analysis, including extensive research methods section

Editorial Reviews

Reviewer: Mark F. Sanders, PhD (UC Davis College of Biological Sciences)
Description: In the 20-plus years since the human genome was first sequenced, significant advancements in the methods and direction of research have occurred. New research directions include proteomics, transcriptomics, metabolomics, and nutritional genomics. Numerous books have been written on these subjects in the last two decades. This book provides a brief introduction to genomics and proteomics, including information on metabolomics and some background on gene mapping.
Purpose: The purpose of the book is to introduce techniques used in genomic and proteomic analysis to students in biochemistry, biotechnology, and allied areas. It is concise, sometimes overly so, but succeeds in overviewing genomic and proteomic analytical methods to readers. These useful, if limited, goals are shared by several other books that cover similar topics in genomics and proteomics more expansively.
Audience: The book will be most useful to undergraduate students taking their first course in genomics and proteomics, although it may not be adequate to cover the subject matter by itself. It provides a sufficient overview of technical approaches in these two research areas, with stronger coverage in proteomics. The authors each have strong undergraduate teaching experience in the subjects covered and use their technical experience to present the information a readable and understandable format.
Features: The book begins with a chapter introducing genomics and proteomics, followed by a chapter on gene mapping. It then continues with two chapters on analyzing genomes, followed by a chapter on nutrigenomics. Four subsequent chapters present methods in proteomic analysis and the final chapter looks beyond genomics and proteomics to introduce several other "omic" approaches. The six chapters covering genomic and proteomic methods are the core of the book. They describe historically important and more contemporary research methods to give readers a general sense of how these techniques work and how they contribute information. The book falls short in that it does not provide a sufficient illustration package for the technical subject matter, and the writing style is concise to the point of inadequacy at times.
Assessment: This book is useful for the subject matter it covers. It provides a good overview of technical methods in genomics and proteomics, but does not attempt to address the wider knowledge base or the interpretations derived from data gathered using these methods.

Doody's Review Service