Practical Cell Culture Techniques / Edition 1

by Alan A. Boulton, Glen B. Baker, Wolfgang Walz

Overview

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

Product Details

ISBN-13:	9780896033481
Publisher:	Springer-Verlag New York, LLC
Publication date:	09/09/1992
Series:	Neuromethods , #23
Edition description:	1992
Pages:	392
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Basic Techniques for Cell Culturing.- Identification of Cell Types in Neural Cultures.- Environmental Influences on Cells in Culture.- Detection and Analysis of Growth Factors Affecting Neural Cells.- The Role of Cell Adhesion Molecules in Neurite Growth.- Three-Dimensional Organ Culture Systems.- Organotypic Slice Explant Roller-Tube Cultures.- Neurons.- Astrocytes.- Oligodendrocytes.- Brain Capillaries.

Editorial Reviews

...a great deal of useful and well-referenced basic information, as well as...methods for a large number of different techniques ...with extensive prools for specific techniques.-Trends in Neurosciences

From the Publisher

Focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. Neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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