Polysaccharide Degrading Biocatalysts

by Rosana Goldbeck (Editor), Patricia Poletto (Editor)

Overview

The transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis.

Polysaccharide-Degrading Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.

• Describes the role of enzymes in the degradation of polysaccharides to obtain building blocks for biochemical processes
• Covers new tools for enzymatic evolution, research protocols, and process strategies contributing to large-scale applications
• Explores the use of polysaccharide hydrolysis products in the areas of pharmaceuticals, fuels, and food engineering
• Features chapter contributions from international experts

Product Details

ISBN-13:	9780323983150
Publisher:	Elsevier Science
Publication date:	02/15/2023
Series:	Foundations and Frontiers in Enzymology
Sold by:	Barnes & Noble
Format:	eBook
Pages:	456
File size:	35 MB
Note:	This product may take a few minutes to download.

About the Author

Dr. Rosana Goldbeck is presently Associate Professor in the Food Engineering Faculty (FEA) at the University of Campinas (UNICAMP). She graduated in food engineering (2005) at the Federal University of Rio Grande (FURG), with a master’s degree (2008) and PhD (2012) in food engineering from the University of Campinas (UNICAMP), and a part of her PhD was carried out at the Universitat Autònoma de Barcelona (UAB). She held a postdoctoral degree at the Brazilian Laboratory of Bioethanol Science and Technology (CTBE) at the National Center for Research in Energy and Materials (CNPEM). Her experience is mainly in the area of biotechnology and bioengineering with emphasis in fermentative processes, production and purification of enzymes, cloning and expression of heterologous proteins, enzymatic hydrolysis, production of biofuels (bioethanol), and other value-added products (xylooligosaccharides) from agro-industrial residues.
Dr. Patrícia Poletto is Full Research Professor at the Federal University of Santa Catarina in Florianópolis—Brazil, and a collaborating researcher at the Biological Engineering Laboratory focused on fermentative and enzymatic processes. Patrícia completed her bachelor’s degree in food engineering (2007) and her PhD in biotechnology (2015) in the area of production and recovery of by solid-state fermentation. She has been working on chemical, fermentative, and enzymatic methods to convert agro-industrial residues mainly into oligosaccharides.

Table of Contents

1. Plant cell wall polysaccharides: Methodologies for compositional, structural, and physicochemical characterization 2. Genetic modification of plants to increase the saccharification of lignocellulose 3. The diversity of plant carbohydrate hydrolysis in nature and technology 4. State-of-the-art experimental and computational approaches to investigate structure, substrate recognition, and catalytic mechanism of enzymes 5. Pretreatments as a key for enzymatic hydrolysis of lignocellulosic biomass 6. Importance of accessory enzymes in hemicellulose degradation 7. How ligninolytic enzymes can help in the degradation of biomass polysaccharides, cleavage, and catalytic mechanisms? 8. Biochemical and biotechnological aspects of microbial amylases 9. Hydrolysis of complex pectin structures: Biocatalysis and bioproducts 10. Macroalgal polysaccharides: Biocatalysts in biofuel/bioenergy production 11. Mathematical modeling of the enzymatic hydrolysis of polysaccharides: A primer 12. Polysaccharide deconstruction products: Production of bio-based building blocks 13. Polysaccharide degradation for oligosaccharide production with nutraceutical potential for the food industry 14. Carbohydrate-active enzymes in the production of lactose-derived tagatose 15. Immobilized biocatalysts for hydrolysis of polysaccharides 16. Carbohydrate-based economy: Perspectives and challenges

What People are Saying About This

From the Publisher

Offers a thorough overview of polysaccharide degrading biocatalysis and related protocols, empowering researchers to discover and develop new enzyme-based approaches

Editorial Reviews

Reviewer: Peter J. Kennelly, PhD (Virginia Tech)
Description: A large portion of global biomass consists of polysaccharides, polymers, and monosaccharides such as glucose or fructose. One of these polysaccharides, starch, is a major component of the human diet as well as the diet of many animals we use as sources of meat, eggs, and milk. Starch is a glucose polymer, as is cellulose. However, the geometry of the bonds that link the glucose units differs, which is why humans can digest starch but not cellulose, and why cellulose renders wood such a useful construction material. The key to whether we can tap the energy contained in polysaccharides resides in the availability of biocatalysts — enzymes — to degrade them into monomer units. Potential energy sources include not only dietary sources of calories, but also the conversion of cellulosic biomass into "green" fuels. This book, a volume in the Foundations and Frontiers in Enzymology series, is devoted to surveying current knowledge and applications of this family of enzymes, important for both human health and the development of green biofuels and biomaterials.
Purpose: The purpose of the book is to "provide a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering."
Audience: The book is intended for research scientists either actively engaged in or considering the study of polysaccharide-degrading enzymes or their optimization for bioprocessing applications. However, its approach and style are appropriate for students as well.
Features: The book generally approaches topics from a global perspective, discussing the current applications and technology as well as future opportunities and challenges before delving into the details. The breadth of subject matter covered is impressive, ranging from mathematical modeling of enzyme-catalyzed polysaccharide hydrolysis to the future prospect of a carbohydrate-based economy. The illustrations complement the text well but are often scaled too small.
Assessment: This book is a pleasant read, even for non-experts. In terms of readability and coverage, it is a thorough, yet surprisingly concise resource on a topic of interest to students and scientists working in the academic and industrial sectors. It is easy to envision using the book for a survey course on polysaccharide-hydrolyzing enzymes and their applications. Ironically, its stiffest competition may be another volume in the same series: Glycoside Hydrolases: Biochemistry, Biophysics, and Biotechnology, Goyal and Sharma (Elsevier, 2023).

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