Plant Mitochondria: Methods and Protocols

by James Whelan (Editor), Monika W. Murcha (Editor)

Overview

The chapters compiled in this detailed collection outline a number of methods used to study plant mihondria today, starting from the isolation of mihondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mihondria’s unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Plant Mihondria: Methods and Prools serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mihondrial research as it links to so many important aspects of plant cell biology.

Product Details

ISBN-13:	9781493926381
Publisher:	Springer New York
Publication date:	04/25/2015
Series:	Methods in Molecular Biology , #1305
Edition description:	2015
Pages:	303
Product dimensions:	7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

Isolation of Intact Mihondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa.- Determining Mihondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5’ End Maturation.- Mihondrial Run-On Transcription Assay Using Biotin Labeling.- In Vitro RNA Uptake Studies in Plant Mihondria.- In Vitro and In Vivo Protein Uptake Studies in Plant Mihondria.- Plant Mihondrial Proteomics.- Identification of Lysine-Acetylated Mihondrial Proteins and Their Acetylation Sites.- A Flowchart to Analyze Protease Activity in Plant Mihondria.- Activity Measurements of Mihondrial Enzymes in Native Gels.- Activity Assay for Plant Mihondrial Enzymes.- Analysis of Type II NAD(P)H Dehydrogenases.- Assessment of Respiration in Isolated Plant Mihondria Using Clark Type Electrodes.- Micro-Respiration Measurements in Plants.- Improvements to Define Mihondrial Metabolomics Using Non Aqueous Fractionation.- Mihondrial Markers of Programmed Cell Death in Arabidopsis thaliana.- Imaging and Analysis of Mihondrial Dynamics in Living Cells.- Analysis of Plant Mihondrial Function Using Fluorescent Protein Sensors.- In Planta Analysis of Leaf Mihondrial Superoxide and Nitric Oxide.- Databases and Informatics Resources For Analysis of Plant Mihondria.- Expression and Crystallization of the Plant Alternative Oxidase.