Plant Genome Engineering: Methods and Protocols

by Bing Yang (Editor), Wendy Harwood (Editor), Qiudeng Que (Editor)

Overview

This volume provides readers with a collection of the latest prools used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Prools is a valuable tool for researchers interested in learning more about this developing and important field.

Product Details

ISBN-13:	9781071631300
Publisher:	Springer US
Publication date:	03/31/2023
Series:	Methods in Molecular Biology , #2653
Edition description:	2023
Pages:	388
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

PAM-Less CRISPR-SpRY Genome Editing in Plants.- Type I-D CRISPR System-Mediated Genome Editing in Plants.- CRISPR/LbCas12a-Mediated Genome Editing in Soybean.- Base Editing in Poplar through an Agrobacterium-Mediated Transformation Method.- Genetic Engineering of Potato (Solanum tuberosum) Chloroplasts using the Small Synthetic Plastome “Mini-Synplastome”.- Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout using CRISPR-BETS.- Construction of CRISPR/Cas9 Multiplex Genome Editing System in Rice.- Use of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants.- Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies.- Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line.- Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector .- Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.).- Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves.- Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.- Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method.- Efficient Targeted Mutagenesis in Brassica Crops using CRISPR/Cas Systems.- Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.).- A CRISPR/Cas9 Prool for Target Gene Editing in Barley.- Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration.- Stepwise Optimization of Real-Time RT-PCR Analysis.- CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding.- Recent Advances in Engineering of In Vivo Haploid Induction Systems.