Plant Chromosomes: Laboratory Methods

Plant Chromosomes: Laboratory Methods

Plant Chromosomes: Laboratory Methods

Plant Chromosomes: Laboratory Methods

eBook

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Overview

Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies.
Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.


Product Details

ISBN-13: 9781351424158
Publisher: CRC Press
Publication date: 12/14/2017
Sold by: Barnes & Noble
Format: eBook
Pages: 286
File size: 10 MB

About the Author

Kiichi Fukui, Shigeki Nakayama

Table of Contents

Plant Chromosomes at Mitosis, K. Fukui,Outline of Mitotic Chromosomes,Preparation Method for the Small Chromosome: Enzymatic Maceration/Air Drying (EMA) Method,Preparation Method for the Large Chromosome: Squash Method,Preparation Method for Chromosomal Samples of Woody Plants,Preparation Method for Chromosome Samples of Suspension Cells,Preparation Method for Chromosome Samples of Flowers and Leaves,Permanent Preparation of Chromosome Samples,Plant Chromosomes at Meiosis, P.G. Goicoechea and R. Giraldez,Introduction,Meiotic Synchrony and Meiotic Gradients,Procedures,Plant Chromosomes at Metabolic Phase, K. Taniguchi,Introduction,Morphological Classification by Distribution of Condensed Heterochromatin,Visualizing Condensation,Application of Chromosome Banding Techniques,Polytene Chromosomes, W. Nagl,Introduction,Occurrence and Structure of Polytene Chromosomes in Plants,Preparation,Staining Methods,DNA and Gene Analysis,Digitation and Computer Analysis,Future Challenges,Flow Cytometry and Chromosome Sorting, J.S. Heslop-Harrison and T. Schwarzacher,Introduction,Procedures,Future Applications,Chromosome Dissection and Direct Cloning, Y. Kamisugi and K. Fukui,Introduction,Equipment,Microdissection of Plant Chromosomes,Direct Cloning,Direct-Labeling and In Situ Hybridization,Chromosome Banding Methods, B. Friebe, T.R. Endo, and B.S. Gill,Introduction,Principles of Chromosome Banding,Procedures,In Situ Hybridization, Y. Mukai,Introduction,Advances in In Situ Hybridization,Procedures,Sister Chromatid Exchange and Replication Banding, B. Friebe and F. Cortés,Introduction,Sister Chromatid Differentiation in Second Division Chromosomes,Sister Chromatid Differentiation in Third Division Chromosomes,Replication Banding,Procedures,Replication of Chromosomes, K. Taniguchi and I. Fujishige,Introduction,Principles of Replication
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