Plant Cell Division: Methods and Protocols

by Marie-Cïcile Caillaud (Editor)

Overview

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Plant Cell Division: Methods and Prools, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Product Details

ISBN-13:	9781071617465
Publisher:	Springer US
Publication date:	10/29/2022
Series:	Methods in Molecular Biology , #2382
Edition description:	2nd ed. 2022
Pages:	267
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

1. Methods to Visualize the Actin Cytoskeleton during Plant Cell Division

Marie-Cécile Caillaud

Part I: Mitosis and Cell Cycle Progression

2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana

Ulkar Ahmadli, Michael Sandmann, Joerg Fuchs, and Inna Lermontova

3. A Prool for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips

Leigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, and William F. Thompson

4. Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures

Kateřina Bišová

5. Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission

Veronika Kselíková, Vilém Zachleder, and Kateřina Bišová

Part II: Manipulation of Cell Division

6. Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal Microscopy

Lukas Hoermayer, Jiří Friml, and Matouš Glanc

7. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells

Tamara Jimenez-Gόngora, Huang Tan, and Rosa Lozano-Durán

Part III: Quantification of Cell Division Patterning

8. Kinematic Growth Analysis of Rice (Oryza sativa) Leaf

Jingjing Fang and Xueyong Li

9. Studying Cell Division Plane Positioning in Early-Stage Embryos

Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia

10. Means to Quantify Vascular Cell File Numbers in Different Tissues

Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, and Bert De Rybel

11. Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis

Roya Campos and Jaimie M. Van Norman

12. Quantification of Cell Division Angles in the Arabidopsis Root

Katia Belcram, David Legland, and Martine Pastuglia

Part IV: Imaging and Quantifying Plant Cytokinesis

13. A Quantitative Method for Evaluating Phragmoplast Morphology

Takema Sasaki and Yoshihisa Oda

14. Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during Cytokinesis

Alexandra Deli, Leda-Eleni Tympa, and Panagiotis N. Moschou

15. Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells

Keisho Maeda and Takumi Higaki

16. Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown Algae

Chikako Nagasato, Rina Yonamine, and Taizo Motomura