Neural Repair: Methods and Protocols

by Vardan T. Karamyan (Editor), Ann M. Stowe (Editor)

Overview

This detailed collection explores a diverse range of topics related to neural repair and functional recovery following ischemic stroke. Techniques detailed in this book span from basic to emerging approaches used to evaluate hallmarks of neurorepair, including axonal remodeling and dendritic arborization, plasticity and functional connectivity, angiogenesis, neuroinflammation, and recovering cerebral blood flow. In addition, several chapters focus on rodent stroke models and post-stroke functional evaluation, clinically-relevant therapeutic paradigms and co-morbidities, pharmacotherapy and methods of delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory prools, as well as tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Neural Repair: Methods and Prools serves as an ideal guide for the wide community of researchers interested in neurorepair following stroke and other forms of CNS injury.

Product Details

ISBN-13:	9781071629284
Publisher:	Springer US
Publication date:	01/31/2024
Series:	Methods in Molecular Biology , #2616
Edition description:	1st ed. 2023
Pages:	492
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Rodent Stroke Models to Study Functional Recovery and Neural Repair.- Subcortical White Matter Stroke in the Mouse: Inducing Injury and Tracking Cellular Proliferation.- A Low Budget Photothrombotic Rodent Stroke Model.- Photothrombotic Model to Create an Infarct in the Hippocampus.- Bilateral Carotid Artery Stenosis and Cerebral Blood Flow Outcomes.- Internal Carotid Artery Stenosis: A Surgical Mouse Model to Study Moyamoya Syndrome.- Modeling Distal Middle Cerebral Artery Occlusion in Neonatal Rodents with Magnetic Nanoparticles or Magnetized Red Blood Cells.- In Vivo Imaging of the Structural Plasticity of Cortical Neurons after Stroke.- Measurement of Uninterrupted Cerebral Blood Flow by Laser Speckle Contrast Imaging (LSCI) during the Mouse Middle Cerebral Artery Occlusion Model by an Inverted LSCI Setup.- Multi-Exposure Speckle Imaging for Quantitative Evaluation of Cortical Blood Flow.- Wide-Field Optical Imaging in Mouse Models of Ischemic Stroke.- Post-Mortem Magnetic Resonance Imaging of Degenerating and Reorganizing White Matter in Post-Stroke Rodent Brain.- Quantitative Spatial Mapping of Axons across Cortical Regions to Assess Axonal Sprouting after Stroke.- Quantitative Evaluation of Cerebral Microhemorrhages in the Mouse Brain.- In Vivo Evaluation of BBB Integrity in the Post-Stroke Brain.- High Resolution RNA Sequencing from PFA-Fixed Microscopy Sections.- FACS to Identify Immune Subsets in Mouse Brain and Spleen.- A Guide on Analyzing Flow Cytometry Data Using Clustering Methods and Non-Linear Dimensionality Reduction (tSNE or UMAP).- Co-Culturing Immune Cells and Mouse-Derived Mixed Cortical Cultures with Oxygen-Glucose Deprivation to In Vitro Simulate Neuroinflammatory Interactions after Stroke.- Assessing Depression and Cognitive Impairment Following Stroke and Neurotrauma: Behavioral Methods for Quantifying Impairment and Functional Recovery.- Use of an Automated Mouse Touchscreen Platform for Quantification of Cognitive Deficits after Central Nervous System Injury.- Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use after Stroke.- The Finer Aspects of Grid-Walking and Cylinder Tests for Experimental Stroke Recovery Studies in Mice.- Performing Enriched Environment Studies to Improve Functional Recovery.- Clinically-Applicable Experimental Design and Considerations for Stroke Recovery Preclinical Studies.- Hydrogels and Nanoscaffolds for Long-Term Intraparenchymal Therapeutic Delivery after Stroke.- Reverse Translation to Develop Post-Stroke Therapeutic Interventions during Mechanical Thrombectomy: Lessons from the BACTRAC Trial.- Methods to Study Drug Uptake at the Blood-Brain Barrier Following Experimental Ischemic Stroke: In Vitro and In Vivo Approaches.- Gene Silencing in the Brain with siRNA to Promote Long-Term Post-Stroke Recovery.- Diabetic Rodent Models for Chronic Stroke Studies.- Use of Conventional Cigarette Smoking and E-Cigarette Vaping for Experimental Stroke Studies in Mice.- Middle Cerebral Artery Occlusion in Aged Animal Model.- Acute Ischemic Stroke by Middle Cerebral Artery Occlusion in Rat Models of Diabetes: Importance of Pre-Op and Post-Op Care, Severity of Hyperglycemia, and Sex.- The DOCA-Salt Model of Hypertension for Studies of Cerebrovascular Function, Stroke, and Brain Health.

Editorial Reviews

Reviewer: Clare Timbie, MD, PhD (University of California San Francisco School of Medicine)
Description: This book of protocols is a volume in the Methods in Molecular Biology series. This collection highlights methods for studying neurologic repair and recovery following stroke, largely in rodent models.
Purpose: The book's goal is to cover techniques spanning from molecular to systems-level approaches to understanding stroke recovery and to present applications to studying recovery in other neural injury models. This is important to advance the study of neural recovery across stroke and injury models. The protocols included in this book cover a wide variety of methods with sufficient detail to ensure reproducibility of experimental techniques in the field.
Audience: The audience for this book includes researchers conducting basic science and translational work to study the mechanisms of stroke recovery from a range of perspectives including molecular mechanisms, behavioral testing, and therapeutic targets. This handbook is largely comprised of details on rodent techniques but also includes content describing methods for conducting clinical research with stroke patients. The authors of each protocol are researchers utilizing these techniques and the collection is edited by Dr. Vardan Karamyan and Dr. Ann Stowe, researchers in the field of neural repair.
Features: This book is divided into five sections, most of which contain an introductory chapter providing an overview of the topic and relevant considerations for experimental design, including protocols for rodent stroke models, post-stroke imaging, molecular mechanisms of post-stroke recovery, post-stroke behavioral assays, models of systemic comorbidities of stroke, and investigating therapeutic targets. Within each protocol, there is a description of the technique, the materials required, and detailed step-by-step instructions. Each protocol also includes extensive explanatory notes including technical details on suppliers, storage conditions, and other tips to improve reproducibility. All protocols are written clearly and concisely and include helpful illustrations of the experimental design and images to demonstrate the technique. Each section covers a range of techniques, from widely adopted approaches to newer methods. For example, the section on rodent stroke models includes protocols for photothrombosis, carotid artery stenosis, and magnetized red blood cell techniques. Additional protocols including non-rodent models or further details on applicability to other models would be helpful, as many of these protocols could be applied to other injury models.
Assessment: This book covers an impressive breadth of techniques in studying recovery after stroke with enough technical detail to promote reproducibility. The methods described are also applicable across stroke models and can be adapted to studying other models of neurologic injury, forming a unique and highly useful resource for researchers studying the mechanisms of neural recovery.

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