Mouse Models of Allergic Disease: Methods and Protocols / Edition 1

by Irving C. Allen

Overview

Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Prools, a wide range of expert contributors provide detailed prools for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease. The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo prools to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo prools used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Timely and authoritative, Mouse Models of Allergic Disease: Methods and Prools serves as an essential collection of prools that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.

Product Details

ISBN-13:	9781627034951
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/14/2013
Series:	Methods in Molecular Biology , #1032
Edition description:	2013
Pages:	326
Product dimensions:	7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

Transgenic Mouse Models.- Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture.- Evaluation of T Cell Function in Allergic Disease.- Evaluating B-Cells: From Bone Marrow Precursors to Antibody Producing Cells.- Prools for Identifying, Enumerating and Assessing Mouse Eosinophils.- Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages.- Applications of Mouse Airway Epithelial Cell Culture for Asthma Research.- Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases.- Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE.- Prools for the Induction and Evaluation of Systemic Anaphylaxis in Mice.- Contact Hypersensitivity Models in Mice.- Induction of Allergic Rhinitis in Mice.- Induction of Allergic Airway Disease Using House Dust Mite Allergen.- An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airways Disease.- PAMPs and DAMPs in Allergy Exacerbation Models.- Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics.- Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma.- Clara Epithelial Cell Depletion in the Lung.- A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma.- Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings.- Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection.- Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes.- Flow Cytometric Methods for the Assessment of Allergic Disease.- Generation of Bone Marrow and Fetal Liver Chimeric Mice.

Editorial Reviews

This book is a collection of beautifully written chapters that are suitable to explore in-depth use of mouse models to understand allergic disease. … This is a must read book for trainees, clinicians and researchers engaged in the study of allergy related manifestations. Undoubtedly, this book will provide an in-depth understanding regarding the use of mouse in allergic research. Using this invaluable text, various key phenomena behind allergic reactions can be explored.” (Sandeep Kumar, World Allergy Organization, worldallergy.org, May, 2015)

From the Publisher