Mouse Models for Drug Discovery: Methods and Protocols / Edition 1

by Gabriele Proetzel, Michael V. Wiles

Overview

As the drug discovery process shifts more and more toward specifically targeting pathways and molecules, model systems continue to increase in importance, and the mouse, with its versatility, ease of use, and remarkable similarity to the human genome, has clearly risen to the forefront of animal model studies. In Mouse Models for Drug Discovery: Methods and Prools, experts in the field present some background for those less familiar with mice as experimental model platforms as well as a collection of techniques involving general methods as well as specific disease topics such as type 1 and 2 diabetes, cardiovascular disease, arthritis, skin disorders, cancer, the use of behavioral models for depression and anxiety, neurodegenerative diseases, neuromuscular diseases, and infectious diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Mouse Models for Drug Discovery: Methods and Prools will stimulate those not familiar with the power of the mouse and its potential for the drug discovery process, and it will encourage the development of new models and new ways to utilize existing models in order to further the use of this dynamic animal in this vital field.

Product Details

ISBN-13:	9781607610571
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/17/2009
Series:	Methods in Molecular Biology , #602
Edition description:	2010
Pages:	421
Product dimensions:	7.10(w) x 10.10(h) x 1.20(d)

Table of Contents

Preface v

Contributors ix

1 Improving Toxicity Screening and Drug Development by Using Genetically Defined Strains Michael F.W. Festing 1

2 The Sophisticated Mouse: Protecting a Precious Reagent Michael V. Wiles Rob A. Taft 23

3 Genetically Engineered Mouse Models in Drug Discovery Research Rosalba Sacca Sandra J. Engle Wenning Qin Jeffrey L. Stock John D. McNeish 37

4 Engineering the Mouse Genome to Model Human Disease for Drug Discovery Frank Koentgen Gabriele Suess Dieter Naf 55

5 Profiling of Drug Action Using Reporter Mice and Molecular Imaging Gianpaolo Rando Andrea Biserni Paolo Ciana Adriana Maggi 79

6 Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies Derry C. Roopenian Gregory J. Christianson Thomas J. Sproule 93

7 Development of Novel Major Histocompatibility Complex Class I and Class II-Deficient NOD-SCID IL2R Gamma Chain Knockout Mice for Modeling Human Xenogeneic Graft-Versus-Host Disease Steve Pino Michael A. Brehm Laurence Covassin-Barberis Marie King Bruce Gott Thomas H. Chase Jennifer Wagner Lisa Burzenski Oded Foreman Dale L. Greiner Leonard D. Shultz 105

8 Bridging Mice to Men: Using HLA Transgenic Mice to Enhance the Future Prediction and Prevention of Autoimmune Type 1 Diabetes in Humans David V. Serreze Marijke Niens John Kulik Teresa P. DiLorenzo 119

9 Mouse Models of Type II Diabetes Mellitus in Drug Discovery Helene Baribault 135

10 Cholesterol Absorption and Metabolism Philip N. Howles 157

11 Collagen-Induced Arthritis in Mice Lisette Bevaart Margriet J. Vervoordeldonk Paul P. Tak 181

12 Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening John P. Sundberg Kathleen A. Silva Caroline McPhee Lloyd E. King 193

13 Models of Metastasis in Drug Discovery James E. Talmadge 215

14 Methods for Evaluating Effects of an Irinotecan + 5-Fluorouracil/Leucovorin (IFL) Regimen in an Orthotopic Metastatic Colorectal Cancer Model Utilizing In Vivo Bioluminescence Imaging David Surguladze Philipp Steiner Marie Prewett James R. Tonra 235

15 CML Mouse Model in Translational Research Cong Peng Shaoguang Li 253

16 Mouse Models for Studying Depression-Like States and Antidepressant Drugs Carisa L. Bergner Amanda N. Smolinsky Peter C. Hart Brett D. Dufour Rupert J. Egan Justin L. LaPorte Allan V. Kalueff 267

17 Virus-Delivered RNA Interference in Mouse Brain to Study Addiction-Related Behaviors Amy W. Lasek Nourredine Azouaou 283

18 Experimental Models of Anxiety for Drug Discovery and Brain Research Peter C. Hart Carisa L. Bergner Amanda N. Smolinsky Brett D. Dufour Rupert J. Egan Justin L. LaPorte Allan V. Kalueff 299

19 Mouse Models of Neurodegenerative Diseases: Criteria and General Methodology Christopher Janus Hans Welzl 323

20 Neuromuscular Disease Models and Analysis Robert W. Burgess Gregory A. Cox Kevin L. Seburn 347

21 Murine Model of Cutaneous Infection with Streptococcus pyogenes Eva Medina 395

22 Murine Model of Pneumococcal Pneumonia Eva Medina 405

23 Murine Model of Polymicrobial Septic Peritonitis Using Cecal Ligation and Puncture (CLP) Eva Medina 411

Index 417

Editorial Reviews

Reviewer: Karen M Kassel, PhD (University of Kansas Medical Center)
Description: This book details methods for drug delivery and common measurements using mouse and disease models.
Purpose: The intent is to convey the importance of using mouse models to study disease. The book describes several genetically altered strains of mice that are used to study various diseases, including cancer, diabetes, and neurological and other diseases. Methods for drug delivery and common endpoint measurements are described for each mouse model.
Audience: This book will useful to those who are interested in using mouse models in their research, particularly for those diseases discussed in the book. It will not provide significant information for models and/or diseases that are not included, but many of the methods will be useful across diverse areas of research.
Features: Several overview chapters begin the book that discuss using mouse models for drug development, the importance of using and preserving mouse models, and the concepts of how genetically altered mice are made. The remaining chapters provide background information on specific mouse models and the diseases they are used to study, as well as methods and materials needed to perform the desired experiments. Methods include routes of drug administration as well as endpoint measurements that may be needed. Although each chapter details only a limited number of routes of drug administration, there is a wide variety of information throughout the book that will be useful to all investigators needing to treat their mice with drugs, regardless of how the drug should be given. The main problem I saw is that many of the figures are not clear. Some are simply too small to be useful, and others are not sharp enough to depict what's in the picture. This can be remedied by making some figures larger and providing others in color. Chapter 14 is an example of figures that are easy to see and understand. In addition, it may be useful to provide more pictures of the different routes of drug administration — both actual pictures of the method being performed on a mouse (particularly for injections, gavage, etc.) and drawings/depictions of the anatomy of the mouse and where the needle would be placed. This was done in a couple of chapters, but should be done throughout.
Assessment: This book will be very useful for researchers using mice to study diseases, particularly if they want to use new procedures. The descriptions of how to administer drugs using various methods are presented well. The figures could be better, but the book is helpful.

Doody's Review Service

From the reviews:

“This book details methods for drug delivery and common measurements using mouse and disease models. … useful to those who are interested in using mouse models in their research, particularly for those diseases discussed in the book. … many of the methods will be useful across diverse areas of research. … useful for researchers using mice to study diseases, particularly if they want to use new procedures. The descriptions of how to administer drugs using various methods are presented well. … the book is helpful.” (Karen M. Kassel, Doody’s Review Service, July, 2010)

From the Publisher