Motion Analysis of Living Cells / Edition 1

by David R. Soll, Deborah Wessels

Overview

Most active cellular functions involve motion. From such subtle subcellular actions as the sorting and targeting of vesicles and organelles leading to the release of hormones to the complex rearrangements of a sphere of embryonic cells forming the body plan of a complete organism, motility of cells is exhibited along a wide range of observable behaviors. Despite this phenomenon, however, the views of modern biology historically have been based on static images and models constructed from biochemical evidence.

Motion Analysis of Living Cells is the first volume to compile the latest research by prominent specialists in molecular and cell biology, presenting newly developed techniques for studying cellular motions and critical analyses of the information these techniques can yield. Focusing on actin-based motility systems and drawing on the latest advances in the techniques of biochemistry, biophysics, microscopy, computer-assisted motion analysis, and molecular genetics, the works in this collection will prove invaluable to future research in embryogenesis, cancer, and diseases related to the cellular immune system.

Among the topics covered:

• Bacterial motility and chemotaxis
• New technologies for characterizing motility-related parameters
• Methods for analyzing the cortical tension of normal cells compared to abnormal cells
• Model for how a cell extends a pseudopod
• Computer-assisted analyses of cytoskeletal mutants of Dictyostelium discoideum
• Emerging methods for studying how cells respond to topographical cues at the substratum
• Polymerization of host actin to facilitate propulsion
• Genetic approaches to the regulatory mechanisms involved in early zebra fish morphogenesis and specification of cell fates
• Specific examples of single cell motility in embryogenesis
• Research oriented toward understanding cancer cell arrest, extravasation, and migration

A groundbreaking treatment of motion analysis of animal cells, Motion Analysis of Living Cells is a thorough review for professionals as well as a comprehensive introduction for students and researchers in the field. It is must reading for cell and developmental biologists, microbiologists, immunologists, neuroscientists, cancer researchers, zoologists, and basic researchers in reproductive medicine.

Product Details

ISBN-13:	9780471159155
Publisher:	Wiley
Publication date:	11/24/1997
Series:	Techniques in Modern Biomedical Microscopy , #3
Pages:	312
Product dimensions:	7.24(w) x 10.28(h) x 0.71(d)

About the Author

David R. Soll is a Professor of Biology at the University of Iowa. He is best known for the motion analysis of living cells, the discovery of Candida albicans phenotypic switching and monoclonal antibody technology. Deborah Wessels is the editor of Motion Analysis of Living Cells, published by Wiley.

Table of Contents

Bacterial Motility and Chemotaxis (J. Armitage & H. Packer).

Two- and Three-Dimensional Computer Systems for Analyzing How Animal Cells Crawl (D. Soll & E. Voss).

Toward the Automated Dissection of Morphological and Molecular Dynamics of Living Normal and Transformed Cells (K. Guiliano, et al.).

Forces and Mechanical Properties in Cell Locomotion (E. Elson, et al.).

The Biochemistry of Animal Cell Crawling (J. Condeelis).

Computer-Assisted Characterization of the Behavioral Defects of Cytoskeletal Mutants of Dictyostelium discoideum (D. Wessels & D. Soll).

Topographical Control of Cell Migration (A. Curtis & C. Wilkinson).

Movement of Bacterial Pathogens Driven by Actin Polymerization (D. Fung & J. Theriot).

Morphogenetic Cell Behaviors and Specification of Cell Fate During Early Teleost Development (M. Cooper & C. Kimmel).

Neural Crest Migration (C. Krull & M. Bronner-Fraser).

Neuronal Growth Cone Motility: Contributions from Neurogenetic Analyses of Cultured Drosophila Neurons (C. Wu).

In Vivo Videomicroscopy of Experimental Hematogenous Metastasis: Cancer Cell Arrest, Extravasation, and Migration (I. MacDonald, et al.).

Index.

Editorial Reviews

Reviewer: Rex Chisolm, PhD (Northwestern University Feinberg School of Medicine)
Description: This book of contributed chapters from leaders in the field of cell motility provides an excellent snapshot of the state of knowledge of the mechanisms responsible for movement of cells and the methods available to study this fundamentally important biological process.
Purpose: The goal is to compile the latest research results and techniques for analyzing cell movement in living cells. Much of our current understanding of cell movement has been derived from static studies, but recently developed technology has made possible the real time imaging and subsequent analysis of the movement of cells and specific cellular compartments and molecules. This book is an excellent starting point to learn about these major advances. One strength is that it effectively interweaves descriptions of the experimental approaches with reviews of the mechanisms derived from these studies.
Audience: Written for anyone with an interest in cell motility, it will be useful for the established investigator in cell movement, students working in the area, and for any biologist desiring to learn the state-of-the-art of how cells move.
Features: It contains many useful illustrations and, as is appropriate for the topic, the static images are often supplemented by Internet addresses where video sequences demonstrating the described motions can be seen.
Assessment: Because of the importance of cell movement to disease processes and the important recent technological breakthroughs, this book is likely to be a very important resource that should be part of any biomedical research collection.

Doody's Review Service

4 Stars! from Doody

This book of contributed chapters from leaders in the field of cell motility provides an excellent snapshot of the state of knowledge of the mechanisms responsible for movement of cells and the methods available to study this fundamentally important biological process. The goal is to compile the latest research results and techniques for analyzing cell movement in living cells. Much of our current understanding of cell movement has been derived from static studies, but recently developed technology has made possible the real time imaging and subsequent analysis of the movement of cells and specific cellular compartments and molecules. This book is an excellent starting point to learn about these major advances. One strength is that it effectively interweaves descriptions of the experimental approaches with reviews of the mechanisms derived from these studies. Written for anyone with an interest in cell motility, it will be useful for the established investigator in cell movement, students working in the area, and for any biologist desiring to learn the state-of-the-art of how cells move. It contains many useful illustrations and, as is appropriate for the topic, the static images are often supplemented by Internet addresses where video sequences demonstrating the described motions can be seen. Because of the importance of cell movement to disease processes and the important recent technological breakthroughs, this book is likely to be a very important resource that should be part of any biomedical research collection.

Rex Chisolm

A dozen papers synthesize recent research in molecular and cell biology and describe newly developed techniques for studying cellular motions and analyzing the information those techniques can yield. They focus on actin-based motility systems and the behavioral phenotype that is the end point in the sequence of the various chemical, structural, and physical processes that contribute to cells moving. Among the topics are bacterial motility and chemotaxis, new technologies for characterizing motility-related parameters, analyzing the cortical tension of normal and abnormal cells, a model for how a cell extends a pseudopod, computer-assisted analyses of cytoskeletal mutants of discoideum, and the polymerization of host actin to facilitate propulsion. Annotation c. by Book News, Inc., Portland, Or.

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