Molecular Bacteriology: Protocols and Clinical Applications / Edition 1

by Neil Woodford, Alan Johnson

Overview

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Prools and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed prools for a range of such methods . We believe that the use of these prools should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed prools for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

Product Details

ISBN-13:	9780896034983
Publisher:	Springer-Verlag New York, LLC
Publication date:	04/30/1998
Series:	Methods in Molecular Medicine , #15
Edition description:	1998
Pages:	682
Product dimensions:	5.98(w) x 9.02(h) x 0.24(d)

About the Author

Woodford, Neil (Central Public Health Laboratory, London); Johnson, Alan P. (Central Public Health Laboratory, London)

Table of Contents

Impact of Molecular Methods on Clinical Bacteriology.- Genomic DNA Digestion and Ribotyping.- Pulsed-Field Gel Electrophoresis.- Plasmid Analysis.- DNA Amplification.- Arbitrarily Primed PCR Methods for Studying Bacterial Diseases.- Genomic Fingerprinting by Application of rep-PCR.- Molecular Approaches to the Identification of Strepocci.- Pneumococcal Diseases.- Molecular Approaches in Mycobacterium tuberculosisand Other Infections Caused by Mycobacterium Species.- Diagnosis and Epidemiology of Diphtheria.- Diagnosis and Epidemiology of Infections Caused by Legionella spp..- Molecular Methods for Haemophilus influenzae.- The Impact o Molecular Techniques on the Study of Meningococcal Disease.- Gonorrhea.- Chancroid.- Mycoplasma and Ureaplasma Infections.- Application of Molecular Methods to the Study of Infections Caused by Salmonella spp..- Cholera.- Diagnosisand Investigation of Diarrheagenic Escherichia coli.- Campylobacter Infections.- Detection and Typing of Helicobacter pylori.- Nosocomial Infections Caused by Staphylococci.- Application of Molecular Techniques to the Study of Nosocomial Infections Caused by Enterococci.- Molecular Approaches for the Detection and Identification of—-Lactamases.- Biochemical and Enzyme Kinetic Applications for the Characterization of—Lactamases.-—Lactam Resistance Mediated by Changes in Penicillin-Binding Proteins.- The Application of Molecular Techniques for the Study of Aminoglycoside Resistance.- Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria.- Quinolone Resistance.- Resistance to Tetracyclines, Macrolides, Trimethoprim and Sulfonamides.

Editorial Reviews

Reviewer: Eugene M. Muller, PhD (Framingham State College)
Description: This is a collection of articles that discuss the molecular biological methods that now, or soon will, affect the fields of clinical microbiology and infectious diseases and bring about significant changes.
Purpose: The publication of this manual is timely; it is a readable review of the most important techniques and their application for the study of the major bacterial pathogens of humans. In the cases in which detailed protocols are not presented, sufficient literature citations are provided.
Audience: Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories.
Features: The introductory chapter discusses the impact of molecular methods on bacteriology; six chapters specifically discuss the current methodologies in use (ribotyping, pulsed-field gel electrophoresis, plasmid analysis, DNA amplification and polymerase chain reaction, genomic fingerprinting) ; seventeen chapters address the major groups of bacterial diseases of humans; and seven chapters address antibiotic resistance. Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations.
Assessment: This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academic communities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases.

Doody's Review Service

"The book is authoritative, as its many contributors are all experts in their fields; their contributions have been well coordinated by the editors, resulting in a unified work. . .The book is sturdily bound in hardback to withstand the considerable use it deserves and will undoubtedly get. It represents very good value and I recommend that all laboratories interested in molecular diagnosis in bacteriology should buy a copy of it."-Journal of Medical Microbiology

"Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories...Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations....This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academic communities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases."-Doody's Health Science Book Review Journal

". . .a book that will be useful not only to the experienced researchers, but also to the newcomer."-Laboratoriums Medizin

From the Publisher

4 Stars! from Doody

This is a collection of articles that discuss the molecular biological methods that now, or soon will, affect the fields of clinical microbiology and infectious diseases and bring about significant changes. The publication of this manual is timely; it is a readable review of the most important techniques and their application for the study of the major bacterial pathogens of humans. In the cases in which detailed protocols are not presented, sufficient literature citations are provided. Although intended primarily for clinical microbiologists and infectious disease specialists, this book will be an excellent reference for investigators who want to use established molecular techniques in a variety of studies. The numerous contributing authors are well regarded within their respective fields and their descriptions and interpretations of their techniques will allow readers to efficiently establish state-of-the-art methodologies in their laboratories. The introductory chapter discusses the impact of molecular methods on bacteriology; six chapters specifically discuss the current methodologies in use (ribotyping, pulsed-field gel electrophoresis, plasmid analysis, DNA amplification and polymerase chain reaction, genomic fingerprinting) ; seventeen chapters address the major groups of bacterial diseases of humans; and seven chapters address antibiotic resistance. Each chapter contained in this volume is current and well written, and supported with an extensive and recent list of citations. This is an important reference for molecular techniques that will soon change clinical microbiology. It will be a valuable addition to the collection of libraries that serve medical and academiccommunities. It is an essential addition to the private libraries of clinical microbiologists and those studying infectious diseases.

Eugene M. Muller

This book provides a wide range of techniques for the diagnosis and investigation of bacterial infections. In addition to the diagnostic and treatment procedures, some of the 31 contributions also show how molecular methods can be applied to different bacterial pathogens, diseases, and antimicrobial resistances. The Notes sections included at the ends of most of the chapters should prove to be of particular interest, as they often include tricks of the trade that the various contributors have learned through personal experience. Annotation c. by Book News, Inc., Portland, Or.

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