Mitochondrial DNA: Methods and Protocols / Edition 3

by Matthew McKenzie

Overview

This third edition is comprised of well-established prools that are considered the gold standard in the field as well as new methodologies for mihondrial DNA analysis. of Mihondrial DNA: Methods and Prools describes prools for detecting mutations in mihondrial DNA, techniques to assess mihondrial DNA damage, visualization of mihondrial DNA in situ, detection of mihondrial DNA nucleoids within the mihondria, methods for analyzing mihondrial DNA replication, mihondrial DNA-encoded protein translation and mihondrial DNA copy number, the latest technologies for modifying the mihondrial genome and methods for the purification of proteins involved in the replication and transcription of mihondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mihondrial DNA: Methods and Prools, Third Edition will be useful not only for mihondrial researchers but also for scientists studying human diseases where mihondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.

Product Details

ISBN-13:	9781493949441
Publisher:	Springer New York
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #1351
Edition description:	Softcover reprint of the original 3rd ed. 2016
Pages:	234
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Comprehensive Mihondrial Genome Analysis By Massively Parallel Sequencing.- Analysis Of Translesion DNA Synthesis By The Mihondrial DNA Polymerase g.- Quantification Of DNA Damage By Real-Time Qpcr.- Quantitation Of Mihondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule PCR.- A Single-Cell Resolution Imaging Prool Of Mihondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity.- Scalable Isolation Of Mammalian Mihondria For Nucleic Acid And Nucleoid Analysis.- Tracking Mihondrial DNA In Situ.- Analysis Of Replicating Mihondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis.- Translation And Assembly Of Radiolabelled Mihondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mihondria.- Analysis Of Mihondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA.- Engineered Mtzfns For Manipulation Of Human Mihondrial DNA Heteroplasm.- Generation Of Xenomihondrial Embryonic Stem Cells For The Production Of Live Xenomihondrial Mice.- Import Of Fluorescent RNA Into Mihondria Of Living Cells.- Purification And Comparative Assay Of The Human Mihondrial Replicative DNA Helicase.- Expression And Purification Of Mihondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster.- Purification And Comparative Assay Of Human Mihondrial Single-Stranded DNA-Binding Protein.- Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mihondrial DNA Replisome.