Microwave Techniques and Protocols / Edition 1

by Richard T. Giberson, Richard S. Demaree Jr.

Overview

Microwave technology can significantly reduce sample turnaround times, enhance quality, and reduce hazardous wastes when compared to bench or automated methods. In Microwave Techniques and Prools, Richard Giberson and Richard Demaree, Jr., have collected a wide range of these time-saving techniques for processing biological samples for evaluation by many different microscopic methods. Described in step-by-step detail by hands-on researchers, these readily reproducible prools include both optimized classic methods and such state-of-the art techniques as in vivo labeling, formalin fixation of fresh tissue, vacuum processing, and processing for scanning electron microscopy. Each stand-alone microwave method has been handcrafted by a researcher who regularly uses it to ensure processing success and the highest quality result.

Comprehensive and time saving, Microwave Techniques and Prools demonstrates for anyone processing biological samples for immunocyhemistry, decalcification, and light or electron microscopy that microwave technology is a critically important tool in all experimental and clinical research laboratories today.

Product Details

ISBN-13:	9781617372452
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/05/2010
Edition description:	Softcover reprint of hardcover 1st ed. 2001
Pages:	220
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Overview of Microwave-Assisted Tissue Processing for Transmission Electron Microscopy.- Vacuum-Assisted Microwave Processing of Animal Tissues for Electron Microscopy.- Vacuum-Microwave Combination for Processing Plant Tissues for Electron Microscopy.- Basic Procedure for Electron Microscopy Processing and Staining in Clinical Laboratory Using Microwave Oven.- Specimen Preparation for Thin-Section Electron Microscopy Utilizing Microwave-Assisted Rapid Processing in a Veterinary Diagnostic Laboratory.- Microwave Processing of Archived Pathology Specimens for Ultrastructural Examination.- Microwave Fixation of Rat Hippocampal Slices.- Microwave Processing Techniques for Biological Samples in a Service Laboratory.- Microwave-Accelerated Decalcification.- Microwave Processing of Sediment Samples.- Microwave Polymerization in Thin Layers of London Resin White Allows Selection of Specimens for Immunogold Labeling.- In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei.- Microwave-Assisted Cyhemistry.- Microwave-Assisted Immunoelectron Microscopy of Skin.- Microwave Paraffin Techniques for Botanical Tissues.- Microwave-Assisted Formalin Fixation of Fresh Tissue.- Microwave-Assisted Processing of Biological Samples for Scanning Electron Microscopy.

Editorial Reviews

"...if you study archived pathology sections, rat hippocampal slices, decalcification, sediments, Allium and Drosophilia nuclei, motor endplates or skin, there is something for you here. Highly specialized therefore, but invaluable if you need this procedure." -Ultramicroscopy

"Microwave Techniques and Prools is both informative, providing a broad perspective on how microwave treatment of samples is being used, and useful as a procedural manual for performing microwave sample treatment and for developing your own techniques." -Journal of Chemical Neuroanatomy

From the Publisher